Relationship between human parvovirus B19 infection and Kikuchiª²Fujimoto disease

    ZHANG Weiª²Ping1, HUANG Gaoª²Sheng1, WANG Juanª²Hong1, WANG Wenª²Qing1, WANG Zhe1, HU Peiª²Zhen1, ZHANG Wei2

    1Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China, 2Department of Pathology, Tangdu Hospital, Fourth Military Medical University, Xi¬ðan 710038, China

    ¡¾Abstract¡¿ AIM: To explore the relationship between parvovirus B19 infection and idiopathic Kikuchiª²Fujimoto disease (KFD). METHODS: Tissue blocks from lymph nodes of 32 idiopathic KFD patients and from normal lymph nodes of 13 normal controls were detected for parvovirus B19 DNA and viral proteins by in situ hybridization and immunohistochemical techniques in a doubleª²blind manner. In situ hybridization and immunohistochemical doubleª²staining were used to determine the type of virusª²infected cells. RESULTS: In situ hybridization and immunohistochemistry revealed that the positive rates of B19 DNA and viral proteins were 71.9% and 59.4% respectively in the 32 idiopathic KFD patients. In the control group, the positive rates were 30.8% and 23.1%, respectively. Statistical analysis indicated that the positive rates of B19 DNA and proteins differed significantly between the idiopathic KFD patients and the controls (P=0.011 and P=0.027 respectively). The B19 virusª²infected cells were mainly lymphocytes and a small number of histocytes. CONCLUSION: Lymph nodes from idiopathic KFD patients have higher parvovirus B19 infection rates than those from controls. Parvovirus B19 infection may play an important role in the pathogenesis of idioª²pathic KFD. ......