基因治疗_____](基础医学)

自制阳离子脂质体介导的体内外基因转移研究

http://www.100md.com 《海军医学杂志》 2005年第2期

基因治疗,,],脂质体；基因转移；肿瘤坏死因子α；基因治疗,1材料和方法,2结果,3讨论,[参考文献]

     [摘要]目的：研究阳离子脂质体介导体内外基因转移的效率。方法：采用逆相蒸发法制备出包裹TNFα DNA的脂质体，将其与体外培养的靶细胞共育，或将其直接注射至小鼠腹腔内，检测TNF α的分泌水平。结果：TNF α基因转染NIH3T3细胞和腹腔细胞，10 d后均可检测到TNF α的分泌。结论：研制的脂质体不但能将目的基因转移至体外培养的靶细胞中，还可通过腹腔内注射直接将TNF α基因转移至腹腔细胞，稳定表达TNF α。

    [关键词] 脂质体；基因转移；肿瘤坏死因子α；基因治疗

    Study on the in vitro and in vivo cytokine gene transfer mediated by selfmade cationic liposome

    LI Mao， GAO Jun， LONG Jianqiu，et al

    (Naval Medical Research Institute, Shanghai 200433，China)

    Abstract: Objective:To investigate the efficiency of cationic liposome in mediating gene transfer. Methods: With reversephase evaporation, we prepared cationic liposome encapsulated TNFα DNA, which were either cocultured with those in vitro cultured target cells, or were directly injected into the abdominal cavity of mice. Then, TNFα secretion level was measured. Results: TNFα secretion could be detected in the NIH3T3 cells and the peritoneal cells transfected with TNFα genes, 10 days later. Conclusion: Results show that liposome can mediate cytokine gene transfer efficiently in vitro and in vivo, and TNFα can be expressed stably. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/04/25/96/84/54.htm

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