摘要 :目的 建立真核细胞表达的大鼠FasL基因重组慢病毒载体三质粒包装细胞系统，为进一步研究转FasL基因在同种异体器官移植中诱导免疫耐受、保护移植物的作用奠定基础。 方法 制备完整的重组慢病毒载体三质粒系统:转移质粒(pLO134-FasL)，包装质粒(ΔNRF)及包膜蛋白质粒(VSV-G)。脂质体法将三质粒共转染包装细胞293T，72h后收集病毒上清，Western-blot法检测293T细胞的FasL蛋白表达。 结果 转染后的293T细胞表达FasL蛋白。 结论 成功建立重组慢病毒载体的三质粒包装细胞系统。

    关键词 :FasL;基因表达;慢病毒载体;293T细胞

    Establishment of the three-plasmid packaging cell line for recombinant lentiviral vector encoding rat FasL gene

    LI Wang，SUN Xiao-qing，HU Shu-qun，et al

    (Department of Urology，Affiliated Hospital of Xuzhou Medical College，Xuzhou，Jiangsu221002，China)

    Abstract:Objective To establish the three-plasmid packaging cell line of the recombinant lentiviral vector encoding rat FasL gene for eukaryotic expression to meet the requisition for deep study on the effect of FasL transgene on inducing immune tolerance and protecting allografts in organic transplantation.Methods The three-plasmid recombinant lentiviral vector，which was made up of the vector plasmid(pLO134-FasL)，the packaging plasmid(ΔNRF)and the envelop plasmid encoding the vesicular stomatitis virus-G glycoprotein(VSV-G) ......