摘要 :目的 探讨不同剂量链脲佐菌素(STZ)致SD大鼠糖尿病肾病模型状况的差异。 方法 58只雄性SD大鼠随机分为5组，模型组大鼠(4组，每组12只)腹腔一次性注射40、50、60、70mg.kg的STZ以建立糖尿病模型;非糖尿病对照组10只，仅予注射缓冲液。注射后24、72h检测血糖，8周观察糖尿病大鼠尿微量白蛋白、血尿素氮、肾脏指数及肾组织光镜和超微结构等变化。 结果 腹腔注射STZ60mg.kg成模率高，模型死亡率较低。8周后大鼠尿微量白蛋白水平即升高，肾脏超微结构检查系膜细胞增生、基质增多、肾小球基底膜增厚。 结论 腹腔注射STZ60mg.kg是致糖尿病SD大鼠发生肾病的最佳剂量。

    关键词 :链脲佐菌素;糖尿病肾病;动物模型;剂量

    Optimal dosage of streptozotocin for inducing diabetic nephropathy in model rats

    LI Wei，ZHANG Hong，YIN Song-lou，et al

    (Department of Endocrinology，Affiliated Hospital of Xuzhou Medical College，Xuzhou，Jiangsu221002，China)

    Abstract:Objective To investigate the differences in diabetic nephropathy model rats induced by streptozotocin(STZ)of varied dosage.Methods 58male SD rats were randomly divided into4diabetic groups(n=12each)and1control group(n=10)to receive varied doses of intraperitoneal STZ and buffer solution respectively.The blood sugar and general conditions were monitored1-3days after the injection.Eight weeks later，the urinary microalbumin，blood urea nitrogen，kidney index and the ultrastructure of kidneywere examined.Results The success rate of model makingwas relatively high and the mortality was relatively low in the STZ60mg.kg group.After8weeks ......