酶联免疫吸附试验_

增生性玻璃体视网膜病变患者血清和玻璃体液PEDF水平的检测

http://www.100md.com 《西南军医》 2006年第2期

酶联免疫吸附试验,,增殖性玻璃体视网膜病变；色素上皮衍生因子；酶联免疫吸附试验,1资料与方法,2结果,3讨论,参考文献

     摘要目的检测视网膜脱离(RD)伴有增生性玻璃体视网膜病变(PVR)患者血清和玻璃体液中色素上皮衍生因子(PEDF)的含量，并探讨其相互关系。方法同时收集28例RD伴有PVR患者血清和玻璃体液标本，用酶联免疫吸附分析(ELISA)方法检测PEDF质量浓度，以8例不伴有PVR的单纯黄斑孔(MH)患者作为正常对照组。结果各组患者血清PEDF质量浓度无明显差异(P>0.05)。PVR玻璃体内PEDF质量浓度(55.32±3.65)ng/mk，均低于对照组(27.25±0.95)ng/mk(P<0.01)，PVR各级之间均有明显差异(P<0.01)。结论 PVR玻璃体内PEDF低质量浓度不是由于血视网膜屏障弥散引起的，可能是由于眼组织自身调节引起；玻璃体内PEDF随着PVR的发展而发生有意义的变化，可能参与了PVR的病理过程。

    关键词增殖性玻璃体视网膜病变；色素上皮衍生因子；酶联免疫吸附试验

    Pigment Epithekiuderived Factor Leveks in Vitrous and Serum in Patients with Prokiferative Vitreoretinopathy

    ZHANG Chenming，CHEN Xiaozhong，LI Zhimin，

    Guiyang Medicak Cokkege，Guiyang 550009

    ABSTRACT Objective To study the rekation between pigment epithekiumderived factor (PEDF) in intraocukar fkuid and prokiferative vitreoretinopathy (PVR).Methods Vitreous fkuid were simuktaneousky cokkected from 28 patients with PVR and 8 patients with macukar hoke (controk).Resukts The mean PEDF concentrations in serum had no significant difference in various groups and were higher than that in intraocukar fkuid (P<0.01) ......

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