摘要 :目的 克隆肾癌G250抗原蛋白多糖(PG)区cDNA，为制备G250杂交瘤及抗G250的单克隆抗体奠定基础。 方法 从肾癌细胞786-0中提取总RNA，以RT-PCR方法扩增出全长360bp的G250抗原PG区cDNA，将其与表达载体pCA13连接，转化大肠杆菌JM109，构建G250抗原PG区cDNA克隆。 结果 酶切及序列分析表明，与GenBank报道的G250抗原PG区cDNA序列完全一致。 结论 G250抗原PG区cDNA已成功地得到克隆。

    关键词 :G250抗原;PG区蛋白;cDNA克隆;序列分析

    Cloning and sequencing of the cDNA of PG region protein of G250antigen

    ZHENG Dian-bao，ZHENG Jun-nian，HAO Lin，et al

    (Department of Urology，Affiliated Hospital of Xuzhou Medical College，Xuzhou，Jiangsu221002，China)Abstract:Objective To obtain the cDNA of proteoglycan(PG)region protein of G250antigen to get ready for preparing the hybridoma and monoclonal antibody of G250antigen.Methods The total RNA was extracted from human renal carcinoma cell line786-0and the intact cDNA of PG region protein was amplified by RT-PCR.The cDNA was cloned into pCA13vec-tor and transformed into E.coli JM109.Results The sequence determined was completely consistentwith the published PG re-gion protein cDNA.Conclusion PG region protein cDNA has been cloned successfully. ......