诱饵载体__(期刊论文)

NDRG家族成员酵母双杂交诱饵载体的构建，表达及自激活检测

http://www.100md.com 《第四军医大学学报》 2005年第5期

诱饵载体,,NDRG；诱饵载体；酵母双杂交；自激活作用,1材料和方法,2结果,3讨论,【参考文献】

     Construction and expression of bait vector for NDRG family members and detection of their selfactivation in yeast twohybrid system

    ZHANG Jing, ZHANG Jian, LIU Na, WANG LiFeng, LI Xia, LIU XinPing, YAO LiBo

    Department of Biochemistry and Molecular Biology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To construct NDRG family bait vectors in yeast twohybrid system and to detect their selfactivation in this system. METHODS: Fulllength cDNA of NDRG family members were obtained by PCR and the fragments of NDRG family members were inserted into PGBKT7 vector according to the reading frame. The bait vectors were transformed into the yeast strain AH109 by PEG/LiAc method and their selfactivation was tested by both the phenotype assay and the color assay. RESULTS: The bait vectors of NDRG family members were constructed correctly and their expressions in yeast were verified. Except for NDRG4B, the other NDRG family members could not activate the reporter gene. CONCLUSION: Apart from NDRG4B, NDRG1, 2 and 3 all can act as baits in the screening of cDNA library to trap the interaction proteins by using yeast two hybrid. ......

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