Construction and expression of mouse discoidin domain receptor 2 fused with enhanced green fluorescent protein gene

    ZHOU Jie1,LIU Xinª²Ping2,ZHANG Yuanª²Qiang1

    1Department of Histology £¦ Embryology,2Department of Biochemistry £¦ Molecular Biology,School of Basic Medicine,Fourth

    Military Medical University,Xi¬ðan 710033,China

    ¡¾Abstract¡¿ AIM: To construct DDR2/EGFP fusion gene in eukaryotic expression vector and to investigate its expression in living cells.METHODS: Mouse discoidin domain receptor 2 (DDR2) was amplified by RTª²PCR and was inserted into pEGFPª²N3.The recombinant expression plasmid DDR2/pEGFPª²N3 was transiently transfected into COSª²7 cells.RTª²PCR and Western Blot were used to evaluate the expression of the fused gene.RESULTS: The results of RTª²PCR and Western Blot showed that DDR2/EGFP fusion protein was properly expressed in COSª²7 cells.CONCLUSION: We have successfully constructed DDR2/EGFP fusion gene in eukaryotic expression vector.This fusion protein has the characteristics of mouse DDR2 and EGFP and can be used as a potential tool for further study on DDR2 functions. ......