Effect of bone marrow stem cells mobilization on heart function of acute myocardial ischemic rats

    WU Feng1,WANG Haiª²Chang1,WANG Yueª²Min2,ZHANG Rongª²Qing1,LI Weiª²Jie1,QIN Tao1,LI Hu1

    1Department of Cardiovasology,Xijing Hospital,2Department of Physiology,School of Basic Medicine,Fourth Military Medical University,Xi¬ðan 710033,China

    ¡¾Abstract¡¿ AIM: To explore the modification of heart function and the possible mechanism by mobilizing the bone marrow stem cells in acute myocardial ischemic rats.METHODS: Myocardial ischemia of left ventricle was produced in male Wistar rats by ligating the left coronary artery.The rat models were randomly divided into 2 groups.Animals in mobilization group received hypodermic injection of rhGª²CSF 20 ¦Ìg/kg and rhª²SCF 200 ¦Ìg/kg,twice per day,continuously for 6 days,and those in control group received hypodermic injection of an equivalent amount of normal saline for 6 days.After 28 days,heart function was examined and myocardial infarct size was determined by dual staining with triphenylterazolium chloride and Evan¬ðs blue.RESULTS: ¢Ù Left ventricular end diastolic pressure (LVEDP) of mobilization group [(22ª±1¡À0ª±7) kPa]was higher than that of control group [(20ª±9¡À0ª±9) kPa],and ¡ÀLVdp/dtmax of mobilization group [(590¡À15) kPa/s and(440¡À18) kPa/s] was statistically higher than that of control group[(567¡À18) kPa/s and (407¡À20) kPa/s] (P<0ª±05);¢Ú Myocardial ischemic size and infarct size of mobilization group [(41¡À7)% and (43¡À6)%]were statistically smaller than those of control group [(53¡À8)% and (55¡À10)%] (P<0ª±05).CONCLUSION: Gª²CSF and SCF can improve the heart function of acute myocardial ischemic rats. ......