骨髓成纤维样基质细胞_

HFCL细胞下调HL60细胞cxcr4基因的表达及意义

http://www.100md.com 《第四军医大学学报》 2005年第5期

骨髓成纤维样基质细胞,,骨髓成纤维样基质细胞；急性髓细胞性白血病；分化；cxcr4,1材料和方法,2结果,3讨论,【参考文献】

     Expression of cxcr4 in HL60 cells directly cocultured with HFCL cells and its clinical significance

    LIANG Rong1, HUANG GaoSheng2, WANG Zhe2, ZHANG YongQing1, WANG JuanHong2, WANG WenQing2, ZHANG WeiPing2, BAI QingXian1, CHEN XieQun1

    1Department of Hematology, Xijing Hospital, 2Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To study the effect of human bone marrow fibroblastoid stromal cells (HFCL) on the expression of cxcr4 gene in acute myeloid leukemia HL60 cells. METHODS: The cell cycle was analyzed by flow cytometry and the cell differentiation was determined by morphologic observation of the ability of NBT cells and flow cytometric detection of expression of CD11b, CD14, CD13 and CD33. The expression of cxcr4 mRNA was tested by semiquantitative RTPCR and Northern blot. RESULTS: The NBT positive cells and the expression of CD11b and CD14 increased. After in direct contact with HFCL cells for 96 h, the mRNA expression levels of cxcr4 genes were downregulated. CONCLUSION: HFCL cells can induce monocytic differentiation in HL60 cells and weaken the ability of metastasis and invasion of HL60 cells. ......

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