HFCL细胞下调HL60细胞cxcr4基因的表达及意义
闂佺ǹ绻戞繛濠囧极椤撶姵瀚氱€广儱绻掔粣锟�骨髓成纤维样基质细胞,,骨髓成纤维样基质细胞;急性髓细胞性白血病;分化;cxcr4,1材料和方法,2结果,3讨论,【参考文献】
Expression of cxcr4 in HL60 cells directly cocultured with HFCL cells and its clinical significance
LIANG Rong1, HUANG GaoSheng2, WANG Zhe2, ZHANG YongQing1, WANG JuanHong2, WANG WenQing2, ZHANG WeiPing2, BAI QingXian1, CHEN XieQun1
1Department of Hematology, Xijing Hospital, 2Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China
【Abstract】 AIM: To study the effect of human bone marrow fibroblastoid stromal cells (HFCL) on the expression of cxcr4 gene in acute myeloid leukemia HL60 cells. METHODS: The cell cycle was analyzed by flow cytometry and the cell differentiation was determined by morphologic observation of the ability of NBT cells and flow cytometric detection of expression of CD11b, CD14, CD13 and CD33. The expression of cxcr4 mRNA was tested by semiquantitative RTPCR and Northern blot. RESULTS: The NBT positive cells and the expression of CD11b and CD14 increased. After in direct contact with HFCL cells for 96 h, the mRNA expression levels of cxcr4 genes were downregulated. CONCLUSION: HFCL cells can induce monocytic differentiation in HL60 cells and weaken the ability of metastasis and invasion of HL60 cells. ......
您现在查看是摘要页,全文长 11393 字符。
限于服务器压力,网站部分信息只供爱心会员或有一定积分的注册会员流览。
此信息需要 2 积分(免费注册登录后每天可以领取10个积分)。
用户名: |
|
密 码: |
|
忘了密码 |
如果您还不是100md.com会员,欢迎 免费注册,
如果您想获得积分,点击这里 查看积分规则。
如果您想订购杂志,请直接与《第四军医大学学报》编辑部联系。
閻庣敻鍋婇崰鏇熺┍婵犲洤妫橀柛銉㈡櫇瑜帮拷
闂佺ǹ绻楀▍鏇㈠极閻愮儤鍎岄柣鎰靛墮椤庯拷
闁荤姴娲ょ€氼垶顢欓幋锕€绀勯柣妯诲絻缂嶏拷
闂佺懓鍚嬬划搴ㄥ磼閵娾晛鍗抽柡澶嬪焾濡拷
|