Protective effects of Alarelin on cisplatinª²induced ovarian toxicity in female rats

    GUO Hongª²Yu,GAO Yunª²He,MA Xiaoª²Ling£¬LI Shuª²Lan

    Sencond Affiliated Hospital£¬Lanzhou Medical College,Lanzhou 730000,China

    ¡¾Abstract¡¿ AIM: To investigate the protective effect of Alarelin on cisplatinª²induced ovarian toxicity in female rats.METHODS: Forty 12ª²weekª²old female Wistar rats [body mass (245ª±6¡À15ª±8) g ] were randomly divided into four groups.Group A received cisplatin (2 mg/kg Qd¡Á10) intraperitoneally,Group B received Alarelin (5 ¦Ìg Qd¡Á10) subcutaneously before the administration of cisplatin (2 mg/kg Qd¡Á10),Group C received only Alarelin (5 ¦Ìg Qd¡Á20 ) subcutaneously,and Group D,used as normal controls,received intraperitoneal administration of 9 g/L sodium chloride.Blood samples were collected from the heart and the levels of blood follicle stimulating hormone (FSH) and estradiol (E2) were determined.Both ovaries and uterus of each rat were removed to measure the amount and the maturity of follicles.Body mass and histomorphological features of the rats were also observed.RESULTS: Compared with that in control group,the mass of ovary and uterus decreased (P<0ª±0001) and a significant reduction was observed in the number of ovarian follicles at each grade (P<0ª±0001).The level of E2 significantly lowered (P<0ª±0001) and the levels of FSH markedly ascended in Group A.Compared with that in Group A,the amount of primitive follicles significantly increased in group B (P<0ª±0001) and Alarelin showed markedly protective effect on the ovarian and uterine morphological construction of rats.CONCLUSION: Alarelin can improve the histomorphologic features of ovary and uterus in pathologic rats.Alarelin has some protective effect on cisplatinª²induced ovarian toxicity in rats. ......