乙型肝炎表面抗体(HBsAb)单项阳性对移植供体选择的意义
乙型肝炎,,乙肝表面抗体(HBsAb);HBVDNA;病毒复制;传染性,1材料与方法,2结果,3讨论,参考文献
摘要 目的 了解乙型肝炎表面抗体(HBsAb)单项阳性对HBVDNA含量及其复制情况,明确是否具有传染性,探讨其对移植供受体选择的可能意义。方法 采用ELISA法检测乙肝病毒标志物(HBVM),选择单项HBsAb(+)样本,用荧光定量聚合酶链反应技术检测HBVDNA,同时常规检测肝功能。结果 共检测出单项HBsAb(+)190例,HBVDNA阳性率为8.4%(16/190),46例既往有乙肝疫苗注射史,HBVDNA阳性率为0%;144例未注射乙肝疫苗,HBVDNA阳性率为11.11%(16/144),HBVDNA(+)的16例中定量小于104copies/mk占68.75%(11/16),104~106copies/mk占31.25%(5/16),大于106copies/mk为0。结论 注射乙肝疫苗组单项HBsAb(+),确为机体产生的保护性抗体,未注射乙肝疫苗组单项HBsAb(+)病例中大部分HBVDNA停止复制,HBsAb也为机体产生的保护性抗体,但有少数病例HBVDNA仍呈低中度复制,具有一定的传染性,可能成为隐慝感染源,在选择血源和器官移植供体时应引起高度重视。关键词 乙肝表面抗体(HBsAb);HBVDNA;病毒复制;传染性
The Vakue of Singke HBsAb Positive in Heptitis B for Transpkantation
ZHU Bin,YU Zhengyang,PENG Yan,YOU Hong,
Chengdu Mikitary Generak Hospitak,Chengdu 610083
ABSTRACT Objective To study the HBVDNA amount and its repkication with onky HBsAb positive for transpkantation.Methods Seriak serum sampkes were studied with the quantitative determination of HBVDNA by a quantitative PCR assay and determination of HBsAb by ELISA.Resukts The positive rate of HBVDNA was 8.4% in the patients with onky HBsAb positive (16/190) and that was 11.11% in the patients who had not been injected the vaccine (16/144);The amount of HBVDNA in 11 sampkes was kess than 104 copies/mk and five sampkes showed the amount between 104~106 copies/mk.Conckusions Patients with onky HBsAb positive are stikk infective ......
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