Barrett食管和食管腺癌的基因表达谱研究
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陈威震, 王 雯
Barrett食管;食管腺癌;表达谱,陈威震,王雯,陈威震,通讯作者,ChangesofgeneexpressioninBarrettesophagusa
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陈威震, 王雯, 南京军区福州总医院消化内科 福建省福州市 350025
陈威震, 2003年南方医科大学硕士生, 主要从事胃肠动力障碍性疾病的研究.
通讯作者: 王雯, 350025, 福建省福州市, 南京军区福州总医院消化内科. wangw68@yahoo.com
电话: 0591-83727698
收稿日期: 2006-04-07 接受日期: 2006-04-22
Changes of gene expression in Barrett esophagus and adenocarcinoma of esophagus
Wei-Zhen Chen, Wen Wang
Wei-Zhen Chen, Wen Wang, Department of Gastroenterology, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, Fujian Province, China
Correspondence to: Wen Wang, Department of Gastroenterology, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, Fujian Province, China. wangw68@yahoo.com
Received: 2006-04-07 Accepted: 2006-04-22
Abstract
AIM: To investigate the changes of gene expre-ssion in Barrett esophagus and adenocarci-noma of esophagus.
METHODS: A dataset of gene expression profilings for 24 paired samples of normal esophageal epithelium, Barrett esophagus and adenocarcinoma of esophagus were downloaded from database GEO and analyzed with Dchip software. The dataset recovered to raw scanned image was firstly verified. Then the gene expression profilings were subjected to gene and sample clusterings to identify the contaminated samples. Finally the genes selected by pair-wise t-tests were reviewed for function.
RESULTS: The quality of GeneChip hybridizations was good, and the percentages of contaminated and cross-hybridized probe sets were both below 5%. The gene and sample clusterings showed that most of the GeneChips were grouped perfectly except N8 and A5 which were located in wrong clusters. Excluding the two tissue contaminated GeneChips, the others were subjected to pair-wise t-test. Twenty-four genes were finally selected, of which 5 were progressively up-regulated in Barrett esophagus and adenocarcinoma of esophagus, while 19 down-regulated. The newly discovered PITX1 that was step-wise down-regulated has been confirmed by a recent study ......
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