RNA干扰抑制HepG2-N10细胞系中HBV基因表达及复制
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潘金水, 任建林, 程 通, 董 菁, 黄松洁,
乙肝病毒;RNA干扰;病毒复制,RNA干扰抑制HepG2-N10细胞系中HBV基因表达及复制,潘金水,任建林,董菁,施华秀,程通,黄松洁,潘金水,通讯作者,RNAinterferenceinhibit
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潘金水, 任建林, 董菁, 施华秀, 厦门大学附属中山医院消化内科 福建省厦门市 361004
程通, 福建省分子医学病毒研究中心, 厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室 福建省厦门市 361004
黄松洁, 厦门大学附属中山医院厦门市临床检验中心 福建省厦门市 361004
潘金水, 在读硕士研究生, 厦门大学附属中山医院消化内科主治医师, 主要从事消化道肿瘤分子生物学研究.
通讯作者: 任建林, 361004, 福建省厦门市, 厦门大学附属中山医院消化内科. jianlinr@msn.com
电话: 0592-2292017 传真：0592-2292017
收稿日期: 2006-02-22 接受日期: 2006-03-15
RNA interference inhibits hepatitis B virus gene expression and replication in HepG2-N10 cells
Jin-Shui Pan, Jian-Lin Ren, Tong Cheng, Jing Dong, Song-Jie Huang, Hua-Xiu Shi
Jin-Shui Pan, Jian-Lin Ren, Jing Dong, Hua-Xiu Shi, Department of Gastroenterology, the Affiliated Zhongshan Hospital of Xiamen University, Xiamen 361004, Fujian Province, China
Tong Cheng, Fujian Research Center of Medical Molecular Virology, Key Laboratory of Cell Biology and Tumor Cell Engineering of Ministry of Education, Xiamen University, Xiamen 361002, Fujian Province, China
Song-Jie Huang, Xiamen Center of Clinical Laboratory, the Affiliated Zhongshan Hospital of Xiamen University, Xiamen 361004, Fujian Province, China
Correspondence to: Professor Jian-Lin Ren, Department of Gastroenterology, the Affiliated Zhongshan Hospital of Xiamen University, Xiamen 361004, Fujian Province, China. jianlinr@msn.com
Received: 2006-02-22 Accepted: 2006-03-15
Abstract
AIM: To evaluate the effect of vector-based small interfering RNA promoted by U6 (pSilencer2.0-U6) on the inhibition of hepatitis B virus (HBV) replication in HepG2-N10 cells.
METHODS: Several sequences targeting on different regions of HBV genome were inserted into pSilencer vectors. The expression plasmids were then transfected into HepG2-N10 cells, a cell line which stably expressed HBsAg, HBeAg and adw2 subtype Dane Particles. Viral antigens were measured by enzyme linked immunosorbent assay (ELISA) ......
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