复方中药益肝康抑制肝星状细胞增殖的作用机制
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张亚平, 姚希贤, 刘晓玲, 赵 霞
益肝康;JNK,AP-1信号转导通路;白细胞介素-1β;肝星状细胞;增殖,张亚平,姚希贤,赵霞,刘晓玲,张亚平,通讯作者,InhibitionofYigan
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张亚平, 姚希贤, 赵霞, 河北医科大学第二医院消化内科 河北省石家庄市 050000
刘晓玲, 沧州市中西医结合医院内科 河北省沧州市 061001
张亚平, 2003级河北医科大学博士生, 副主任医师, 目前主要从事肝纤维化研究.
通讯作者: 姚希贤, 050000, 河北省石家庄市, 河北医科大学第二医院消化内科. gracezhangyaping@yahoo.com.cn
电话: 0311-87814356
收稿日期: 2006-02-16 接受日期: 2006-03-20
Inhibition of Yigankang on proliferation of rat hepatic stellate cells induced by interleukin-1β
Ya-Ping Zhang, Xi-Xian Yao, Xiao-Ling Liu, Xia Zhao
Ya-Ping Zhang, Xi-Xian Yao, Xia Zhao, Department of Gastroenterology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China
Xiao-Ling Liu, Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Cangzhou 061001, Hebei Province, China
Correspondence to: Dr. Xi-Xian Yao, Department of Gastroenterology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China. gracezhangyaping@yahoo.com.cn
Received: 2006-02-16 Accepted: 2006-03-20
Abstract
AIM: To study the mechanism of Yigankang in the proliferation inhibition of rat hepatic stellate cells (HSCs).
METHODS: The activation of JNK pathway was detected by Western blot, while the proliferation of HSCs was induced by interleukin-1β (IL-1β). The effect of JNK inhibitor SP600125 was measured by cell counting kit-8 (CCK-8) and AP-1 activity was evaluated by electrophoretic mobility shift assay.
RESULTS: Interleukin-1β up-regulated the proliferation of HSCs. After stimulation of IL-1β for 24 h, HSC proliferation increased significantly as compared with that in the controls (1.573 ± 0.026 vs 1.390 ± 0.073, P = 0.000). After treatment with different concentrations of SP600125 (10 µmol/L, 1.427 ± 0.113; 20 µmol/L, 0.772 ± 0.093; 40 µmol/L, 0.675 ± 0.074), HSC proliferation induced by IL-1β decreased significantly in comparison with that in the controls (1 ......
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