NS-398联用AG-041R对胃癌细胞的协同抑制作用机制
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朱 峰, 孙为豪, 李淑德, 李兆申
NS-398;AG-041R;胃癌;细胞增殖;凋亡,朱峰,李淑德,李兆申,孙为豪,朱峰,通讯作者,Synergisticinhibitory
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朱峰, 李淑德, 李兆申, 中国人民解放军第二军医大学附属长海医院消化科 上海市 200433
孙为豪, 南京医科大学第一附属医院老年医学科 江苏省南京市 210029
朱峰, 第二军医大学2004级在读博士.
通讯作者: 朱峰, 200433, 上海市, 中国人民解放军第二军医大学附属长海医院消化科. zfyzj@hotmail.com
收稿日期: 2006-02-17 接受日期: 2006-03-07
Synergistic inhibitory effect of NS-398 combined with AG-041R on gastric cancer cells
Feng Zhu, Wei-Hao Sun, Shu-De Li, Zhao-Shen Li
Feng Zhu, Shu-De Li, Zhao-Shen Li, Department of Gastroenterology, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China
Wei-Hao Sun, Department of Geriatrics, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Correspondence to: Feng Zhu, Department of Gastroenterology, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China. zfyzj@hotmail.com
Received: 2006-02-17 Accepted: 2006-03-07
Abstract
AIM: To investigate the inhibitory effects and mechanisms of combined treatment of a special COX-2 inhibitor (NS-398) and a special gastric receptor antagonist (AG-041R) on gastric cancer cell line MKN-45.
METHODS: The human gastric cancer cell line MKN-45 was treated with NS398 and AG-041R, respectively, or in combination. The growth of MKN-45 cells was detected by MTT assay; the cell apoptosis was detected by flow cytometry; the expression of c-Myc mRNA was determined by reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS: NS-398 and AG-041R inhibited the growth of MKN-45 cells in a time- and dose-dependent manner (12-72 h; NS-398: 1 × 10-8 – 1 × 10-4 mol/L; AG-041R: 1 × 10-8 – 1 × 10-5 mol/L). AG-041R (1×10-6 mol/L) and NS-398 (1×10-5 mol/L) inhibited the proliferation of MKN-45 cells at rates of 42.1% and 41.8%, respectively, at 72 h. NS-398 and AG-041R in combination resulted in a synergistic effect on inhibiting growth, and the inhibition effect was enhanced along with time ......
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