PTTG antisense cDNA enhances sensitivity of gallbladder cancer cells to 5ª²FU

    DOU Guoª²Rui1, WANG Jiang2, ZHANG Fuª²Qin2

    1Brigade of Cadets, 2Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To investigate the effect of PTTG antisense cDNA on the sensitivity of gallbladder cancer cells to 5ª²FU. METHODS: The recombinant vector containing PTTG antisense cDNA was transfected into GBCª²SD gallbladder cancer cells. The positive cell clones were screened by G418. PTTG protein expression was detected by Western blot analysis. The changes of the cell proliferation and cell relative viability after treatment with different concentrations of 5ª²FU were assessed by MTT, the doseª²effects curves were obtained and IC50s were calculated. Flow cytometry was used to demonstrate discrepancy of apoptotic rates. RESULTS: The expression of PTTG protein in transfected cells was decreased significantly as compared with that in nonª²transfected ones. The cell proliferation was accelerated and the cell apoptotic rate was higher in transfected cells. The IC50 of 5ª²FU was significantly lower in transfected cells (0.605 mmol/L) than in nonª²transfected and empty vector transfected cells (1.117 mmol/L and 0.914 mmol/L respectively). After treatment with 5ª²FU, the apoptotic ratio was significantly higher in transfected cells (26.24%) than in nonª²transfected and empty vector transfectedcells (2.67% and 7.62% respectively). CONCLUSION: PTTG antisense cDNA transfection enhances the sensitivity of gallbladder cancer cells to 5ª²FU. Such an enhancement of sensitivity suggests that the combination of PTTG antisense cDNA with 5ª²FU can be an effective treatment modality for gallbladder cancer. ......