膳食诱导肥胖抵抗大鼠下丘脑NPY mRNA及蛋白的表达
膳食配方,,膳食配方；肥胖症；大鼠，近交系；下丘脑；蛋白质类,膳食诱导肥胖抵抗大鼠下丘脑NPYmRNA及蛋白的表达,1材料与方法,2结果,3讨论,参考文献
【摘要】目的 研究膳食诱导肥胖(DIO)及肥胖抵抗(DIO-R)大鼠下丘脑弓状核神经肽Y mRNA及蛋白表达情况，探讨神经肽Y与肥胖抵抗的关系。方法 高脂饮食建立DIO与DIO-R大鼠模型，分别于2周、12周末处死，采用原位杂交和免疫组化方法检测下丘脑弓状核神经肽Y的 mRNA和蛋白表达情况。结果 实验2周末，DIO大鼠神经肽Y mRNA和蛋白阳性面积分别为(0.563 8±0.100 5),(0.610 9±0.143 4)mm2，高于DIO-R(0.411 5±0.085 1)，(0.523 1±0.096 3)mm2和对照组大鼠(0.400 4±0.062 2)，(0.445 3±0.157 8)mm2；神经肽Y mRNA和蛋白积分光密度分别为9 025.48±2 192.32，7 786.39±1 423.02，高于DIO-R(7 651.79±2 279.21，5 447.16±3 207.09)和对照组大鼠(6 499.24±2 342.94，5 530.83±841.13)。结论 神经肽Y mRNA和蛋白表达降低且保持敏感性可能与肥胖抵抗有关。【关键词】 膳食配方；肥胖症；大鼠，近交系；下丘脑；蛋白质类
Study on the Neuropeptide Y mRNA and Protein Expression of Dietinduced Obesity Rats
ZHAI Lingling, YAO Xingjia, ZHAO Jian, et al.
Department of Child and Adolescent Health, College of Public Health, China Medical College,Shenyang (110001), China
【Abstract】Objective To investigate NPY mRNA and protein expression in the arcuate nucleus of hypothalamus of dietinduced obesity(DIO) and dietinduced obesity resistant(DIOR) rats, and to explore the relationship between NPY and obesityresistant. Methods The DIO and DIOR rat models was established by high fat diet. The rats were killed in 2 and 12 weeks. Situ hybridization and immunohistochemistry were used to measure the expression of NPY mRNA and protein in the rats. Results After 2 weeks of diet, the areas of NPY mRNA and protein expression of DIO rats were(0.563 8±0.100 5)mm2 and(0.610 9±0.143 4)mm2, higher than those of DIOR(0.411 5±0.085 1,0.523 1±0.096 3) and the control rats(0.400 4±0.062 2,0.445 3±0.157 8). The IOD of NPY mRNA and protein expression of DIO were 9 025.48±2 192.32 and 7 786.39±1 423.02, higher than DIO-R (7 651.79±2 279.21,5 447.16±3 207.09) and the control rats (6 499.24±2 342.94,5 530.83±841.13). Conclusion Decreased NPY mRNA and the kept NPY sensitivity might be related to obesityresistant. ......
您现在查看是摘要页，全文长 8238 字符。