Expression of cyclooxygenaseª²2 in cervical carcinoma and its significance

    MA Xiaoª²Dong1, CHEN Biª²Liang1, MA Xiangª²Dong1, ZHAO Yinª²Xiao2, GUO Huiª²Ling1

    1Department of Obstetrics and Gynecology, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China, 2Department of Obstetrics and Gynecology, PLA 537th Hospital, Baoji 721006, China

    ¡¾Abstract¡¿ AIM: To study the significance of COXª²2 expression in the cervical cancer tissues and cell lines. METHODS: Immunohistochemical assay was used to detect COXª²2 in 29 cervical cancer, 14 chronic cervitis and 18 normal cervixes. Immunohistochemistry and in situ hybridization were used to detect the COXª²2 protein and mRNA in cervical cancer cell lines: SiHa and HeLa. The relationship between the expression of COXª²2 and the clinicopathologic parameters of cervical cancer was analyzed. RESULTS: ¢ÙThe overall positive expression rate of COXª²2 in cervical cancer was 75.9%, higher than that in normal cervix and chronic cervitis (P<0.01); ¢ÚThe intensity of expression of COXª²2 in cervical cancer was different in different tumor stages and tumor grades (P>0.05); ¢Û The overall positive expression rate of COXª²2 in cervical squamous carcinoma was higher than that in cervical adenocarcinoma but with no statistical significance (P>0.05), and no expression of COXª²2 was found in cervical adenosquamous carcinoma; ¢Ü The overall positive expression of COXª²2 was observed in HeLa cells and SiHa cells, but the expression intensity was not significantly different; ¢Ý The positive expression of COXª²2 mRNA was found in both HeLa cells and SiHa cells, and the expression intensity was not significantly different. CONCLUSION: The overª²expression of COXª²2 in cervical tumor is related to the onset and progression of the disease. ......