Expression of adhesion molecules in periª²intracerebral hemorrhage foci in rats

    LI Gaiª²Li1, ZHANG Shouª²Xin1, WANG Bingª²Ang2, ZHANG Hongª²Bin1, WANG Baiª²Ren3

    1Department of Neurology, 2Department of Orthopaedics, Third Hospital of Chinese PLA, Baoji 721004, China, 3Institute of Neuroscience, School of Basic Medicine, Fourth Military Medical University, Xi¡¯an 710033, China

    ¡¾Abstract¡¿ AIM: To study dynamic changes of the expression of adhesion molecules in the intracerebral hemorrhage foci and to investigate the pathophysiological processes after intracerebral hemorrhage so as to provide theoretic basis for clinical treatment. METHODS: Collagenase was injected into the striatum of the rats to cause bleeding. Immunohistochemical ABC method was used to observe the expressions of ICAMª²1, VCAMª²1 and Eª²selectin 24, 72 h and 7 d after the intracerebral hemorrhage. RESULTS: Plentiful ICAMª²1ª²like, VCAMª²1ª²like and Eª²selectinª²like immunopositive cells were found in striatum around hemorrhage foci in groups 24 h, 72 h and 7 d after the intracerebral hemorrhage and the numbers were significantly different (P<0.05) from those of the nonª²hemorrhage sides of striatum at the same time point. The expressions of same adhesion molecules around hemorrhage foci were different at different time points. The numbers of the three kinds of adhesion molecules immunopositive cells at the hemorrhage sides in the 24 h group were the greatest [ICAMª²1, VCAMª²1 and Eª²selectin were (152¡À4), (141¡À2) and (131¡À4) mm2, respectively]. The numbers of the three kinds of adhesion molecules immunopositive cells at the hemorrhage sides in the 72 h group [(44¡À3), (98¡À4) and (36¡À5) mm2] and 7 d group [(43¡À3), (96¡À4) and (35¡À3) mm2] all decreased, which were significantly different from those of the 24 h group (P<0.05). However, there was no significant difference in number between the 72 h and the 7 d groups (P>0.05). CONCLUSION: The adhesion molecules are highly expressed in the hemorrhage area, which may be related to the course of intracerebral hemorrhage. ......