Preparation of human selenoprotein P cDNA probe and expression in human hepatic tissue

    NAN Keª²Jun1, SUI Chengª²Guang1, HAN Yue1, LIU Yanª²Fang2, HU Peiª²Zhen2, QIN Haiª²Xia1, JING Zhao1, LIU Shanª²Xi3

    1Department of Oncology, 3Department of Hematology, First Hospital, Xi¡¯an Jiaotong University, Xi¡¯an 710061, China, 2Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xi¡¯an 710033, China

    ¡¾Abstract¡¿ AIM: To investigate the expression of selenoprotein P mRNA in hepatic diseases, including cirrhosis and hepatocellular carcinoma (HCC). METHODS: Selenoprotein P cDNA fragment was amplified by PCR the expression of Selenoprotein P mRNA was detected in 13 cases of normal liver, 20 cases of cirrhosis and 30 cases of hepatocellular carcinoma (HCC) by in situ hybridization. RESULTS: The PCR products evaluated by electrophoresis were as expected. The expression of selenoprotein P mRNA was found in the tissue stroma of liver, especially in the endothelial cells of vasculature. The positive expression rate of selenoprotein P mRNA was 84.6% (11/13), 50.0% (10/20), 20.0% (6/30) respectively in normal liver, cirrhosis and HCC. The rate of expression in hepatic carcinoma was significantly lower than that in others (¦Ö2=15.84, P<0.005; ¦Ö2=4.96, P<0.05). The positive blue granules were seen distributed in the nucleus and cytoplasm in normal liver and cirrhosis liver. The expression in nucleus of normal liver was significantly higher than that of cirrhosis liver. In HCC, the positive particles were located in cytoplasm, but nucleolus showed almost no staining. CONCLUSION: The expression of human selenoprotein P gene is significant different in the three kinds of liver tissues, suggesting that selenoprotein P may play a role in the occurrence and development of HCC. ......