肝星状细胞_____肝星状细胞；乙醛；NFκB；IκB

乙醛调控大鼠HSC中NFκB的表达及其意义

http://www.100md.com 《第四军医大学学报》 2005年第10期

肝星状细胞,,肝星状细胞；乙醛；NFκB；IκB,1材料和方法,2结果,3讨论,【参考文献】

     Expression of NFκB in rat hepatic stellate cells stimulated by acetaldehyde

    GAN XinYu,JIANG MingDe,XIE FangWei,ZENG WeiZheng,WU XiaoLing

    Department of Gastroenterology,General Hospital of Chengdu Military Command,Chendu 610083,China

    【Abstract】 AIM: To investigate the effects of acetaldehyde on the proliferation and the expression of NFκB and IκB in rat hepatic stellate cells (HSCs) and to explore its mechanism.METHODS: HSCs were isolated by means of density gradient centrifugation after digestion in pronase E and collagenase Ⅳ and the relationship between acetaldehyde and the proliferation and the expression of NFκB and IκB in HSCs were studied by MTT,immunohistochemistry and EMSA.RESULTS: ① HSCs were isolated successfully.② The freshly isolated HSCs proliferated significantly after 24 h stimulation with acetaldehyde at a concentration of 200 μmol/L [(221±010) vs (052±005),P<001].③ The expression of IκB was inhibited when it is stimulated by acetaldehyde at a concentration of 200 μmol/L [(032±002) vs (160±032),P<001].④ Thirty minutes after the stimulation of acetaldehyde (200 μmol/L),the expression of NFκB in HSC increased,and 120 min after the stimulation of acetaldehyde (200 μmol/L),the NFκB activity in HSCs reached the peak [(392±082) vs (082±004),P<001].CONCLUSION: Acetaldehyde can inhibit the expression of IκB and activate the expression of NFκB in quiescent HSCs,by both of which the proliferation of HSCs is activated. ......

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