rAAV-AFP转染人外周血单核细胞来源树突状细胞增强免疫刺激功能
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梁 政, 罗荣城, 尤长宣, 郑 航
重组腺相关病毒;甲胎蛋白;树突状细胞;基因转染;肝细胞癌梁政,罗荣城,尤长宣,郑航.rAAV-AFP转染人外周血单核细胞来源树突状细胞增强免疫刺激功能. 世界华人消化杂志2006;14(1
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梁政, 罗荣城, 尤长宣, 郑航, 南方医科大学南方医院肿瘤中心 广东省广州市 510515
梁政, 2005年南方医科大学硕士, 主治医师, 主要从事肿瘤生物治疗的研究.
广东省自然科学基金资助项目, No. 037050
通讯作者: 罗荣城, 510515, 广东省广州市, 南方医科大学南方医院肿瘤中心. center@nfhoc.com
电话: 020-87640966
收稿日期: 2006-03-18 接受日期: 2006-04-12
Infection of rAAV-AFP enhances immunostimulatory effect of human peripheral blood monocyte-derived dendritic cells
Zheng Liang, Rong-Cheng Luo, Chang-Xuan You, Hang Zheng
Zheng Liang, Rong-Cheng Luo, Chang-Xuan You, Hang Zheng, Cancer Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
Supported by the Natural Science Foundation of Guangdong Province, No. 037050
Correspondence to: Professor Rong-Cheng Luo, Cancer Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China. center@nfhoc.com
Received: 2006-03-18 Accepted: 2006-04-12
Abstract
AIM: To study the immunostimulatory effect of rAAV-AFP (recombinant adeno-associated virus expressing α-fetoprotein antigen) transfection on dendritic cells (DCs) derived from human peripheral blood monocytes.
METHODS: Newly isolated dendritic cells were infected with rAAV-AFP. The percentage of viable DCs was observed by trypan blue exclusion every 24 h. After transfection, the alterations of surface markers on mature DCs, including CD80, CD86, CD83, CD40, CD1a, HLA-DR and α-fetoprotein (AFP), were detected by flow cytometry. Meanwhile, 3H-thymidine incorporation method was used to measure the capacity of T-cell proliferation before and after transfection. The specific killing activity of T cells was evaluated by MTT assay.
RESULTS:About 77.7% mature DCs expressed AFP protein. The viable DCs percentages and surface marker expression showed no significant changes after transfection (P > 0.05). Transfected DCs still had strong potential of stimulating the proliferation of T lymphatic cells, and there was no significant difference between transfected and non-transfected group (P > 0 ......
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