多基因甲基化在胰腺癌发病机制中的作用
http://www.100md.com
卜献民, 赵成海, 张 宁, 王 巍, 李 岩,
卜献民,戴显伟,赵成海,张宁,王巍,李岩,通讯作者:,电话:,收稿日期:,接受日期:,Roleofmultiplegenesmethylatio
 |
| 第1页 |
参见附件(849KB，4页)。
卜献民, 戴显伟, 中国医科大学附属二院普通外科 辽宁省沈阳市 110004
赵成海, 张宁, 王巍, 中国医科大学病理生理教研室 辽宁省沈阳市 110001
李岩, 中国医科大学附属二院消化内科 辽宁省沈阳市 110004辽宁省教育厅高等学校科学技术研究项目, No. 05L557
通讯作者: 戴显伟, 110004, 沈阳市和平区三好街36号, 中国医科大学附属二院普通外科. buxianmin@163.com
电话: 024-83956512
收稿日期: 2006-03-14 接受日期: 2006-04-06
Role of multiple genes methylation in pancreatic carcinogenesis
Xian-Min Bu, Cheng-Hai Zhao, Ning Zhang, Wei Wang, Yan Li, Xian-Wei Dai
Xian-Min Bu, Xian-Wei Dai, Department of General Surgery, the Second Affiliated Hospital of China Medical University, Shenyang 110004, Liaoning Province, China
Cheng-Hai Zhao, Ning Zhang, Wei Wang, Department of Pathophysiology, China Medical University, Shenyang 110001, Liaoning Province, China
Yan Li, Department of Gastroenterology, the Second Affiliated Hospital of China Medical University, Shenyang 110004, Liaoning Province, China
Supported by the Fund for University Science and Technology Research from Education Office of Liaoning Province, No. 05L557
Correspondence to: Xian-Wei Dai, Department of General Surgery, the Second Affiliated Hospital of China Medical University, 36 Sanhao Street, Heping District, Shenyang 110001, Liaoning Province, China. daixw@hotmail.com
Received: 2006-03-14 Accepted: 2006-04-06
Abstract
AIM: To determine methylation status of RASSF1A, p16, SOCS-1 and hMLH1 genes and explore the roles of their concurrent methylation in the carcinogenesis of pancreatic cancer.
METHODS: DNA in pancreatic cancer and cancer-adjacent tissues was extracted by saturated NaCl method. The methylation status of these genes was detected by methylation-specific polymerase chain reaction (MSP).
RESULTS: The methylation rates of RASSF1A, p16, SOCS-1 and hMLH1 were 36.4%, 13.6%, 13.6% and 4.5% in cancer-adjacent tissues and 59.1%, 40.9%, 31 ......
您现在查看是摘要介绍页，详见PDF附件(849KB，4页)。