实时荧光定量PCR方法检测幼兔粪便双歧杆菌的实验研究
http://www.100md.com
陈津津, 蔡 威
实时荧光定量PCR;双歧杆菌;粪便,陈津津,蔡威,陈津津,.,通讯作者:,电话:,收稿日期:, 接受日期:,Real-ti
 |
| 第1页 |
参见附件(1011KB，5页)。
陈津津, 蔡威, 上海交通大学医学院附属新华医院临床营养中心 上海市 200092
陈津津, 2004年上海交通大学医学院博士生, 主治医师, 主要从事胃肠道微生态的研究.国家自然科学基金资助项目, No. 30271350
通讯作者: 蔡威, 200092, 上海市控江路1665号, 上海交通大学医学院附属新华医院临床营养中心. caiw204@yahoo.com.cn
电话: 021-65790000
收稿日期: 2006-03-10 接受日期: 2006-03-24
Real-time quantitative PCR procedure for quantification of bifidobacteria in fecal samples from infant rabbits
Jin-Jin Chen, Wei Cai
Jin-Jin Chen, Wei Cai, Center of Clinical Nutrition, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
Supported by National Natural Science Foundation of China, No. 30271350
Correspondence to: Dr. Wei Cai, Center of Clinical Nutrition, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, China. caiw204@yahoo.com.cn
Received: 2006-03-10 Accepted: 2006-03-24
Abstract
AIM: To quantitatively detect the bifidobacteria in the fecal samples from infant rabbits by real-time quantitative polymerase chain reaction (RTQ-PCR).
METHODS: Genus-specific primer of bifidobacteria was designed for 16S rDNA-targeted PCR. The standard curve of RTQ-PCR was generated from a grade-dilution of plasmid DNA which was cloned from PCR products and spectroscopically quantified. Bacterial genome DNA in fecal samples was extracted from the bifidobacteria-fed rabbits and the controls for analysis by RTQ-PCR, respectively.
RESULTS:Bifidobacteria was positive in both groups of rabbit fecal samples. The level of bifidobacteria in the bifidobacteria-fed rabbits was significantly higher than that in the control group (logarithm for the numbers of bifidobacteria per 0.05 g feces: 6.37 ± 0.58 vs 5.18 ± 0.98, P = 0.004).
CONCLUSION: Real-time quantitative PCR can accurately quantify the bifidobacteria in fecal samples from infant rabbits. ......
您现在查看是摘要介绍页，详见PDF附件(1011KB，5页)。