高灵敏化学发光法体外HBV转录与复制水平的检测体系
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何 芳, 唐 红, 刘 丽, 刘凤君, 王 甦,
乙型肝炎病毒;转录;复制;化学发光法;检测,何芳,唐红,刘丽,刘凤君,王甦,周陶友,赵连三,刘聪,何芳,通讯作者:,电话:,
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何芳, 唐红, 刘丽, 刘凤君, 王甦, 周陶友, 赵连三, 刘聪, 四川大学华西医院感染性疾病中心; 生物治疗国家重点实验室四川大学感染病分子生物学研究室; 四川省感染性疾病分子生物学重点实验室 四川省成都市 610041
何芳, 2001年四川大学博士, 讲师, 主要从事病毒性肝炎的基础与临床研究.国家杰出青年科学基金资助项目, No. 30325036
四川省应用基础研究项目, No. 04JY029-002-7
通讯作者: 唐红, 610041, 四川省成都市国学巷37号, 四川大学华西医院感染性疾病中心. htang6198@hotmail.com
电话: 028-85422647 传真: 028-85422113
收稿日期: 2006-03-14 接受日期: 2006-03-24
Establishment of a highly sensitive chemiluminescent detection system for analysis of hepatitis B virus transcription and replication level in vitro
Fang He, Hong Tang, Li Liu, Feng-Jun Liu, Su Wang, Tao-You Zhou, Lian-San Zhao, Cong Liu
Fang He, Hong Tang, Li Liu, Feng-Jun Liu, Su Wang, Tao-You Zhou, Lian-San Zhao, Cong Liu, Center of Infectious Diseases, West China Hospital of Sichuan University; Division of Molecular Biology of Infectious Diseases, National Key Laboratory of Biotherapy; Sichuan Provincial Key Laboratory of Molecular Biology of Infectious Diseases, Chengdu 610041, Sichuan Province, China
Supported by the National Science Fund for Distinguished Young Scholars, No. 30325036, and the Applied Basic Research Programs of Sichuan Province, China, No. 04JY029-002-7
Correspondence to: Professor Hong Tang, Center of Infectious Diseases, West China Hospital, Sichuan University, 37 Guoxue Xiang, Chengdu 610041, Sichuan Province, China. htang6198@hotmail.com
Received: 2006-03-14 Accepted: 2006-03-24
Abstract
AIM: To establish a stable and highly sensitive chemiluminescent detection system for analysis of hepatitis V virus (HBV) transcription and replication level in vitro.
METHODS: Human hepatoma cell line HepG2 and HepG2.2.15, a HepG2 cell line with HBV transcription and replication, were collected after being cultured for 3 d and then divided equally. Total RNA of the cells and HBV replication intermediates were extracted and analyzed by Northern and Southern blot hybridization, respectively, while digoxigenin labeled HBV recombinant plasmid pHBV4 ......
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