毛细管胶束电动色谱_

毛细管胶束电动色谱法测定淫羊藿及其中药制剂中淫羊藿苷的含量

http://www.100md.com 《广东药学院学报》 2000年第2期

毛细管胶束电动色谱,,毛细管胶束电动色谱；淫羊藿苷；药物分析；淫羊藿,1材料和方法,2结果,3讨论,参考文献

     摘要建立淫羊藿及其制剂中淫羊藿苷的毛细管胶束电动色谱分析方法。电泳缓冲液由20 mmol/L四硼酸钠、45 mmol/L脱氧胆酸钠、φ=35%乙腈(pH=8.5)组成，以卡马西平为内标，工作电压25kV，检测波长270 nm。该方法在5～80 mg/L范围内呈线性，r＝0.9998，日内、日间RSD1.9%～4.8%。毛细管胶束电动色谱法专一性强，精密度高，是一种较好的中药有效成分检测方法。

    关键词毛细管胶束电动色谱；淫羊藿苷；药物分析；淫羊藿

    Determination of Icariin in Epimedium and Its preparation by Micellar Electrokinetic Capillary Chromatography

    LIANG Tong，ZhOU Keyuan，LING Guangxin

    (Guangdong Medical College，Zhanjiang Guangdong 524023)

    Abstract A method of micellar electrokinetic capillary chromatography for determination of icariin in epimedium and its preparation was established. The running buffer consisted of 20 mmol/L sodium borate, 45 mmol/L sodium deoxycholate and φ=35% acetonitile. Carbamazepine was used as internal standard.The working voltage was 25 kV, and the detection wavelength was 270 nm. The result showed that there was a linear relationship in the range from 5 to 80 mg/L(r＝0.9998). A relative standard deviation of withinday and betweenday were observed from 1.9% to 4.8%.The method showed good precision and specificity, and was a better method for determination of effective components from Traditional Chinese medicine. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/05/21/97/50/61.htm

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