摘要: 目的 探讨复方高渗盐溶液(高渗氯化钠羟乙基淀粉40注射液，HSH)对失血性休克大鼠脾脏细胞凋亡的影响。方法 采用SD大鼠失血性休克模型，分别用HSH或乳酸林格氏液(RL)复苏，复苏后1、2、4小时处死动物，使用HE染色、透射电镜及TUNEL法观察其脾脏中淋巴细胞凋亡情况。结果 RL组复苏后1小时、HSH组各时相点凋亡指数(AI)与假手术组比较无明显差异(P>0.05 )。HSH组各时相点AI无明显差异(P>0.05)。RL复苏组除复苏后1小时外，AI均明显高于HSH组(P<0.05)。结论 HSH能减少失血性休克诱导的脾脏淋巴细胞过度凋亡，对失血性休克后机体免疫功能紊乱起保护作用。

    关键词:；羟乙基淀粉；失血性休克；脾脏；凋亡；免疫

    Effects of HSH resuscitation on spleen apoptosis in rats following hemorrhagic shock

    WANG Yong,ZHAO Chaoying,QIAN Cheng,et al.

    (Department of General Surgery,Jiangwan Hospital,Shanghai 200434,China)

    Abstract: Objective To evaluate effects of HSH resuscitation on the spleen apoptosis in rats following hemorrhagic shock.Methods Controlled hemorrhagic shock was induced by blood withdrawal(mean arterial pressure,40mmHg) and maintained for 60 min.Animals were randomly divided into 3 groups:group 1 (n=6) was shamoperated,group 2(n=18) was treated with RL,and group 3 treated with 6ml/kg HSH(n=18).The rats were killed 1,2,4h after resuscitation.Use HE staining,Transmission Electron Microscopy,TUNEL staining to detect the apoptosis of lymph cell in spleen ...... 婵犵數濮烽。浠嬪焵椤掆偓閸熷潡鍩€椤掆偓缂嶅﹪骞冨Ο璇茬窞闁归偊鍓涢悾娲⒑闂堟胆褰掑磿閺屻儳鍙曟い鎺嗗亾闂囧鏌ㄥ┑鍡欏缁绢厼鐖奸弻鈩冩媴闂堟稈鍋撳┑瀣祦鐎广儱娲犻崑鎾绘晲鎼粹€茬凹缂備降鍔嶅銊ф閹烘垟妲堟慨妤€妫欐晥缂傚倷妞掔欢銈嗕繆閸ヮ剙绠熼柟缁㈠枛闁卞洭鏌ｉ弬鍨棌婵″樊鍨跺娲传閸曨偅娈舵繝娈垮枤閺佺ǹ鈽夐悽绋块唶闁哄洨鍋犻幗鏇㈡⒑闂堟冻绱￠柛鏇ㄥ幘閺嗩垶姊绘担鐑樺殌闁宦板姂閹囧籍閸屾粎鐣堕梺鍛婁緱閸犳鎱ㄥ⿰鍫熺參婵☆垯璀﹀Σ鍝モ偓娈垮枛濞硷繝寮婚敓鐘茬＜婵﹩鍘鹃妶顐ょ磽娴ｄ粙鍝虹紒璇茬墕閻ｇ兘鏁愭径妯绘櫖濠电娀娼уΛ妤呯嵁鐎ｎ喗鈷戦柛婵嗗椤忔挳鏌涢妸褎鏆柨婵堝仱閺佸啴宕掑槌栧晣婵犵數濮撮敃銈夊箠鎼淬劌纾荤€广儱娲犻崑鎾斥枔閸喗鐏嶅┑鐐插级閿曘垽鐛弽顓炲窛濠电姴鍊荤粣鐐烘煛婢跺﹦澧愰柡鍛矒瀹曟椽宕掗悙瀵稿帗闂侀潧顧€婵″洭鎯屾惔銏㈢闁哄鍨堕ˉ銏ゆ煙椤栨氨校闁瑰嘲鎳庨湁閻庯綆浜欐竟鏇炩攽閻樿尙浠涢柟绋垮⒔缁牊寰勯幇顓炰化闂佹悶鍎洪崜锕€螞閹达附鐓曢悗锝呭缁夘喚鈧娲橀悷褔骞忛悩缁樺殤妞ゆ帊鑳剁粔铏繆閵堝洤啸闁稿鍋涚叅婵☆垱浜介埀顒佸浮閹虫粓鎮界喊妯荤亙闂佽鍑界紞鍡涘磻閸涘瓨鍋傛繛鍡樻尰閻撶喖鏌曟繛鍨姎妞ゅ繈鍊濋弻宥堫檨闁告挻姘ㄩ崚鎺撴償閵娿儲鐎梺褰掓？閻掞箓宕甸崶顒佺厵閻庣數枪瀛濆銈忕到閵堟悂寮诲澶娢ㄩ柨鏂垮⒔閻ｆ椽姊洪幖鐐插Ω闁告梹鍨垮畷褰掑箚瑜夐崼顏堟煕閹扳晛濡介柡瀣⒔缁辨捇宕掑顒佹闂佹悶鍔屽ḿ鈩冧繆鐎涙ê绶為柟閭﹀墰娴煎鈹戦悩缁樻锭妤楊亝鎸冲畷顖濈疀濞戞瑢鎷婚梺绋胯閸婃洟寮搁弮鍫熺厓鐟滄粓宕滈妸鈺佺闁跨噦鎷�


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