染料木黄酮_____染料木黄酮；胰岛β细胞；膜电位

木黄酮对小鼠胰岛β细胞膜电位的影响

http://www.100md.com 《第四军医大学学报》 2005年第20期

染料木黄酮,,染料木黄酮；胰岛β细胞；膜电位,1材料和方法,2结果,3讨论,【参考文献】

     Effect of genistein on membrane potentials of mouse pancreatic βcells in vitro

    ZHAO YuFeng, ZHU YunLong, HU YuZhen, ZHOU JingJun, ZHONG YanQing

    Department of Physiology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】AIM: To determine the effect of longterm inactivation of protein tyrosine kinases on pancreatic βcells. METHODS: Primarily cultured mouse pancreatic βcells were pretreated by 0.1 mmol·L-1 genistein for 12 hours. The membrane potentials of βcells were recorded by using perforated patchclamp techniques with wholecell mode. RESULTS: Genistein treatment significantly decreased the resting membrane potentials of βcells under the condition of both 2 mmol·L-1 glucose and 0.2 mmol·L-1 diazoxide, the opener of KATP channels. After stimulated by 15 mmol·L-1 glucose, normal βcells depolarized with short latent periods and provided the bursts of action potentials. In contrast, mouse βcells treated by genistein had significant increase in latent periods before depolarization, significant attenuation of action potentials, and significant elevation in membrane potentials at interval of action potentials. CONCLUSION: Longterm treatment with genistein impairs the responses of pancreatic βcells to glucose, and it is indicated that decrease in tyrosine kinases activity causes the dysfunction of pancreatic βcells. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/05/21/97/54/45.htm

您现在查看是摘要页，全文长 9207 字符。