关键词：胞二磷胆碱；学习；记忆；morris水迷宫；脑缺血

    摘要：目的 探讨胞二磷胆碱对大鼠局部脑缺血后空间学习和记忆能力的作用。 方法 实验分为正常对照组、缺血对照组和胞二磷胆碱组。线栓法制作大鼠大脑中动脉阻塞(MCAO)模型，术后2周内腹腔注射胞二磷胆碱，第15天开始采用Morris水迷宫装置评价大鼠的空间学习和记忆能力。 结果 脑缺血对照组大鼠在定向航行试验和空间探索试验中均表现出明显的空间认知功能的障碍，胞二磷胆碱组大鼠平均逃避潜伏期与缺血对照组比较明显缩短(P<0.01)。在空间探索试验中，胞二磷胆碱组大鼠原平台象限停留时间百分比以及穿过原平台位置次数均大于脑缺血对照组(P<0.01)。缺血对照组多采用边缘式和随机式搜索策略；正常对照组多采用直线式和趋向式搜索策略；胞二磷胆碱组采用趋向式和随机式搜索策略的频数较多。 结论 胞二磷胆碱可以明显改善大鼠永久性局部脑缺血后空间学习和记忆和能力。

    Effect of citicoline on spatial learning and memory of rats after focal cerebral ischemia

    ZHAO Jian-jun, LIU Yong, CHEN Xin-lin, LIU Jian-xin, TIAN Ying-fang, ZHANG Peng-bo, KANG Qian-yan, QIU Fen

    Research Center for Neuroscience, Department of Anatomy, Histology and Embryology, School of Medicine of Xi'an Jiaotong University, Xi’an 710061, China

    Abstract: Objective To investigate the effects of citicoline on spatial learning and memory of rats after focal cerebral ischemia. Methods The rats were randomly divided into sham-operation group, ischemia control group and citicoline group. In the later two groups, focal cerebral ischemia model was established by introducing an intraluminal filament into the left middle cerebral artery, and citicoline (500 mg/kg) or 0.9% NaCl was administered intraperitoneally once a day for 2 weeks after the operation. The rats in the sham-operation group were not subjected to middle cerebral artery occlusion (MCAO) with intraluminal filament. The spatial learning and memory functions of the rats were evaluated by Morris water maze test 15 days after MCAO for 5 days. Results The rats in ischemia control group exhibited serious spatial learning and memory deficits in both place navigation test and spatial probe test. In the former test, the mean escape latency of citicoline-treated rats were significantly shorter than that of ischemia control rats (P<0.01), and in the latter test significantly diffidence was noted between citicoline group and ischemia control group in the percentage time spent in the former platform quadrant and frequency of crossing the former platform (P<0.05). Conclusion Citicoline can improve the spatial learning and memory function of rats after focal cerebral ischemia. ......