HBeAg结合蛋白4基因剪切体HBeBP4A的克隆化
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张健康, 郭 江, 成 军, 王丹琼, 伦永志,
乙型肝炎病毒e抗原;结合蛋白;克隆化;剪切体;真核表达载体,张健康,郭江,成军,王丹琼,伦永志,赵龙凤,蓝贤勇,洪源,毛羽,张健康,赵龙凤,张健康
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张健康, 郭江, 成军, 王丹琼, 伦永志, 赵龙凤, 蓝贤勇, 洪源, 毛羽, 北京地坛医院传染病研究所 北京市 100011
张健康, 赵龙凤, 山西医科大学第一医院消化科 山西省太原市 030001
张健康, 山西医科大学2004级内科学博士生, 主要从事慢性肝病的临床与基础研究.
国家自然科学基金资助项目, No. 30371288
北京市自然科学基金项目, No. 5042024
通讯作者: 成军, 100011, 北京市东城区安外大街地坛公园13号, 北京地坛医院传染病研究所. cj@genetherapy.com.cn
电话: 010-64481639 传真: 010-64281540
收稿日期: 2006-01-06 接受日期: 2006-01-25
Cloning of spliced variant HBeBP4A of hepatitis B virus e antigen binding protein 4
Jian-Kang Zhang, Jiang Guo, Jun Cheng, Dan-Qiong Wang, Yong-Zhi Lun, Long-Feng Zhao, Xian-Yong Lan, Yuan Hong, Yu Mao
Jian-Kang Zhang, Jiang Guo, Jun Cheng, Dan-Qiong Wang, Yong-Zhi Lun, Long-Feng Zhao, Xian-Yong Lan, Yuan Hong, Yu Mao, Institute of Infectious Diseases, Beijing Ditan Hospital, Beijing 100011, China
Jian-Kang Zhang, Long-Feng Zhao, Department of Gastroenterology, the First Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
Surpported by National Natural Science Foundation, No. 30371288, and Beijing Natural Science Foundation, No. 5042024
Correspondence to: Professor Jun Cheng, Institute of Infectious Diseases, Beijing Ditan Hospital, 13 Ditan Park, Anwai Street, Dongcheng District, Beijing 100011, China. cj@genetherapy.com.cn
Received: 2006-01-06 Accepted: 2006-01-25
Abstract
AIM: To clone a new gene, binding protein 4 spliced variant HBeBP4A of hepatitis B virus e antigen (HBeAg), and to explore its function and structure by bioinformatical analysis.
METHODS: HBeBP4A was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using HepG2 cDNA as template and inserted into pGEM-T easy vector by TA cloning. Recombinant eukaryotic expression vector (pcDNATM3.1/myc-HisA-HBeBP4A) was constructed by subcloning followed by restriction enzyme digestion analysis and sequencing. Bioinformatical methods were used to analyze its possible physical and chemical characteristics, structure, and function ......
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