带血供骨膜瓣对异体骨移植血管化的影响
骨膜瓣,,骨膜瓣；异体骨移植；血管内皮细胞生长因子；微血管密度,1材料与方法,2结果,3讨论,参考文献：
摘要:目的 探讨带血供骨膜瓣对异体骨移植血管化的影响。方法 将兔的股骨经去抗原化后作为供体，制作带血供骨膜瓣包裹同种异体骨修复兔大段骨缺损的模型，实验设置对照组，即未用带血供骨膜瓣包裹移植物。分别在术后 4周、8周和16周 分别对移植物及周围软组织、骨组织进行组织学观察(HE染色),对两组移植物进行免疫组织化学染色，显示内皮细胞生长因子(VEGF)的表达。对Ⅷ因子相关抗原特异性标记，比较不同时间微血管密度(MVD)的变化。结果 实验组骨组织哈佛氏管内较早出现微血管；在相同阶段，实验组VEGF的表达强于对照组，MVD高于对照组。结论 带血供骨膜瓣对异体骨移植的血管化过程有明显的促进作用。关键词:骨膜瓣；异体骨移植；血管内皮细胞生长因子；微血管密度
The Effect of Periost Flap with Bloodsupply on Vascularization in Allogenic Bone Transplantation
ZHANG Feng,TAO Shengxiang
(The Hospital of PuFang Group,Chibi Hubei 437321,China)
ABSTRACT:Objective To study the effect of periost flap with blood supply on vascularization in allogenic bone transplantation. Methods Deantigenic rabbit femoral as the donor, an adult rabbit model of massive femoral defect was established and reconstructed with bone allograft covered by periost flap maintaining bloodsupply,and with nonvascular bone allograft as the control groups. Histological observation (HE stain ) and immunohistochemical examination (VEGF) were done on bone and its surrounding tissues at 4,8,16 weeks postoperatively ......
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