【摘要】 目的 了解早期应用双歧杆菌对非肥胖糖尿病(NOD)小鼠糖尿病发病的影响。方法 将4周龄雌性NOD小鼠随机分为两组：实验组(20只)给予双歧杆菌0.25mg/g体重/天(制备成活菌悬液0.5ml)，对照组(20只)给予等量的磷酸盐缓冲液(PBS)。4周龄开始给药至30周龄，每周测体重，于15周龄时各组分别处死6只小鼠，取胰腺组织HE染色进行胰岛炎评分；其余小鼠30周龄时观察发病率，同时观察胰岛炎，脾脏T细胞亚群变化。结果 双歧杆菌组糖尿病发病率28.57%，明显低于对照组78.57%(P<0.05)，发病时间也显著延缓，胰岛炎程度明显减轻，残存胰岛数目高于对照组；脾CD4+、CD8+T细胞亚群双歧杆菌组明显低于对照组(CD4+T细胞P<0.01,CD8+T细胞P<0.05)。结论 双歧杆菌对NOD小鼠1型糖尿病发病有预防和延迟的作用，其机制可能与调节小鼠CD4+、CD8+T细胞亚群有关。

    【关键词】 双歧杆菌 1型糖尿病 T细胞亚群

    Preventive Function of Bifidobacterium for Type I Diabetes in Female NOD Mice

    Zhang Mei,et al.

    (Biochemistry Department,Qiqihar Medical College,Heilongjiang Province 161042,China)

    【Abstract】 Objective To study whether bifidobacterium can prevent the onset of type 1 diabetes in female non-obese diabetic(NOD) mice.Methods 40 female NOD mice aged four weeks were divided into two groups.Experiment group(n=20)was orally administered bifidobacterium(0.25mg/gBM/d,prepared viable organism suspension 0.5ml),and the contrast group (n=20) was given PBS by the same regimen.Mice aged four weeks were administered until 30 weeks of age.At the age of fifteen weeks,the degree of insulitis was observed；The incidence of diabetes was detected at age of thirty weeks,at the same time,to observe insulitis and change of T cell subsets of spleen.Results Treatment with bifidobacterium reduced the total incidence of diabetes(28.57% Vs 78.57%,P<0.05) and delay its onset compared with control group.The level of insulitis were lessen than control group,but the number of survival areas of Langerhans were higher than control group；CD4+、CD8+T cell subsets of spleen were lower obviously than control group.(CD4+ T cell P<0.01,CD8+T cell P<0.05).Conclusions The data demonstrated the preventive effects of bifidobacterium on the onset of type 1 diabetes and the severity of insulitis in female NOD mice.The mechanisms of these effects may be related to the change of CD4+、CD8+T cell subsets. ......