摘要：目的：为探讨大肠癌手术标本中Skp2的表达及其与p27kip1表达的相关性。方法：应用免疫组织化学STREPT-AVIDIN-BIOTIN-COMPLEX法，检测大肠癌手术标本中Skp2、p27kip1的表达。结果：Skp2的表达的阳性率：浸润于粘膜以及粘膜下层为0%(0/4)，浸润达肌层者为40%(2/5)。浸润达浆膜层者为61%(43/71)；淋巴结转移阳性者阳性率为77%(36/47)；临床病理分期第1、2、3期阳性率分别为22%(2/9)，29%(7/24)和77%(36/47)。Skp2在大肠癌中的表达与p27kip1的表达呈现明显负相关；统计学检验有显著性(P<0.01)。结论：大肠癌组织中， Skp2的过度表达和p27kip1的表达缺失有明显的相关性，可能通过降低p27kip1的表达而促进肿瘤的浸润和转移。检测癌组织中Skp2、p27kip1的表达情况，有助于评估肿瘤的生物学行为，对患者的预后判断具有重要意义。

    关键词： 大肠癌； Skp2； p27kip1； 免疫组织化学

    Expression of Skp2 in Colorectal Carcinomas and its Relation with p27kip1

    XU Shuang-ta, HE Ya-li, XU Jian-hua, et al

    (The Second Affiliated Hospital to Fujian Medical Universty,Fujian Quanzhou 362000, China)

    Abstract： Objective： To analyze the expression of skp2 and p27kip1 in a series of human tissue sample representing different stages of colorectal carcinomas. Method： Paraffin embedded materials from 80 samples of colorectal carcinomas were analyzed by immunohistochemistry for skp2 and p27kip1 expression. Result： Of 80 cases, 45(56.3%) were positive for skp2. The expression of skp2 was significantly related to the lymphatic metastasis77%(36/47) and the rate of skp2 expression in different TNM stage of the colorectal carcinomas. It also has significant relation with the expression of p27kip1 (p<0.01).Conclusion： The skp2 expression are related to the invasion and metastasis of the colorectal carcinomas and the expression of p27kip1.It promotes the invasion and metastasis of colorectal carcinomas . ......