摘要: 通过比较脑肿瘤患者和正常人脑脊液的二维凝胶电泳图谱(two-dimensional electrophoresis，2DE)，并对差异蛋白进行质谱鉴定，以寻找肿瘤特异脑脊液蛋白。以脑肿瘤患者和正常人的脑脊液为研究对象，采用固相pH梯度(immobilized pH gradient，IPG)2DE分离总蛋白质，凝胶经银染显后，用ImageMaster2D图像分析软件进行比较分析、识别差异表达的蛋白质。结果得到肿瘤患者脑脊液蛋白点924个，正常脑组织蛋白点607个，匹配512个，匹配率分别为55.4%和84.3%，去冗余后发现，有35个蛋白点只在脑肿瘤患者脑脊液图谱中出现。肿瘤患者脑脊液和正常对照脑脊液双向电泳图谱有明显差别，但是本实验尚未对这些差异蛋白进行质谱鉴定，所以未找出肿瘤特异蛋白。

    关键词: 脑肿瘤;蛋白质组;蛋白质组学;双向电泳;脑脊液

    Identification of Tumor-related Proteins by Proteomic Analysis of Cerebrospinal Fluid From Patients with Primary Brain Tumors

    ZHANG Xu-hua，LIU Shi-lian，LI Wei

    (Medical College，Shandong University，Jinan250012，China;School of Conotral Science and Engineering，Shandong University，Jinan250014，China)

    Abstract:To find the proteins that present only in tumor CSF by Two-dimensional gel electrophoresis Two-dimensional gel electro-phoresis was used to find proteins only in cerebrospinal fluid(CSF)from patients with tumor and in CSF from patients with other nerve system disorders(OND).Results showed that different2-D maps were received because of the above differences.There were924protein sopts on tu-mor gels and607protein spots on OND gel ......