纤维素酶_____纤维素酶;_木聚糖酶;

β聚糖酶的诱导合成Ⅱ：初始pH值与培养温度的影响

http://www.100md.com 《广东药学院学报》 2003年第4期

纤维素酶,,纤维素酶;,木聚糖酶;,绿色木霉,1材料与方法,2结果与讨论,3结论,参考文献

     摘要以绿色木霉(Trichoderma viride)LH-011为产酶菌株，利用自制复合碳源，对初始pH值、培养温度等因素对β聚糖酶合成的影响进行了研究。结果表明：以预处理玉米芯为碳源(浓度15 g/L)，调节碳氮比为6.4，初始pH 4.8，150 r/min摇瓶培养，前期(32±1) ℃培养24 h，后期(28±1) ℃变温调控培养72 h，纤维素酶活力和木聚糖酶活力分别达到了3.76 U/mL和13.95 U/mL。

    关键词纤维素酶; 木聚糖酶; 绿色木霉

    Biosynthesis of βglycanaseⅡ：effects of initial pH value and cluture temperature

    LI Kunping,PAN Tianling,Huang Keying

    (1. Department of Pharmaceutical Engineering,Guangdong College of Pharmacy,Guangzhou,Guangdong 510240; 2. Industry School,Central South Forestry University,Zhuzhou,Hunan 412006)

    Abstract The effects of initial pH value and culture temperature on βglycanase synthesis by Trichoderma viride LH-011 were studied .15 g/L pretreated corn cob was used as the main corbon source and inducer,mixed (NH4)2SO4 and urea and peptone as nitrogen source. C/N was regulated to 6.4,with the initial pH value of 4.8,at 150 r/min.The temperature was(32±1) ℃ in the first 24 hours and (28±1) ℃ for 48 h. The cellulase and xylanase activity reached 3.76 U/mL and 13.95 U/mL respectively after 72 hours cultivation. ......

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