鱼腥草_____鱼腥草；(期刊论文)

鱼腥草药材中黄酮苷类成分的HPLC指纹图谱研究

http://www.100md.com 《广东药学院学报》 2005年第2期

鱼腥草,,鱼腥草；,HPLC；指纹图谱；中药色谱指纹图谱相似度评价系统,1仪器和试药,2试验方法,3方法学考察,4试验结果,参考文献

     摘要目的建立鱼腥草药材黄酮苷类成分的HPLC指纹图谱。方法采用梯度洗脱法，对鱼腥草药材进行HPLC测定，流动相为甲醇0.1%磷酸线性梯度洗脱，检测波长为320 nm；记录时间：60 min。采用中国药典委员会出版的中药色谱指纹图谱相似度评价系统软件(版本：2004A)进行比较计算。结果运用梯度洗脱能很好的分离鱼腥草的各种成分，通过软件的比较，鱼腥草药材的指纹图谱相似度均大于0.90。结论本文所建立的方法可作为鱼腥草药材中黄酮苷类成分的指纹图谱。

    关键词鱼腥草； HPLC；指纹图谱；中药色谱指纹图谱相似度评价系统

    HPLC for fingerprint analysis of flavone glycosides from Houttuynia cordatd Thunb

    LI Xiaomeng，XU Weiliang，HE Xinrong

    (Department of Natural Medicinal Chemistry, Guangdong Pharmaceutical University, Guangzhou 510240,China)

    A bstract Objective To obtain HPLC fingerprint of flavone glycosides from Houttuynia cordatd Thunb. Methods Houttuynia cordatd Thunb was extracted with methanol in a microwave system. Then, the extracts were analyzed on a HPLC column using gradient elution with methanol0.1% phosphoric acid under the detection wavelength of 320 nm. The spectra were compared with a software “Similarity Evaluation System for Chromatographic Fingerprint of TCM” published by GPC (Version 2004A). Result and Conclusion Flavone glycosides from Houttuynia cordatd Thunb were well separated by HPLC with a high similarity (90%) to the standard, suggesting that this HPLC method could be used to obtain the fingerprint of flavone glycosides from Houttuynia cordatd Thunb. ......

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