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VEGF, bFGF诱导的人外周血单个核细胞向血管内皮祖细胞分化的研究
http://www.100md.com 《第四军医大学学报》 2006年第8期
单核细胞,,单核细胞;内皮祖细胞;诱导,VEGF,bFGF诱导的人外周血单个核细胞向血管内皮祖细胞分化的研究,0引言,1材料和方法,2结果,3讨论,【参考文献】
     Differentiation of peripheral blood mononuclear cells into endothelial progenitor cells in vitro induced by VEGF and bFGF

    SUN BaoHua, WANG Ling, YAO Qing, YI Jun, WANG Hui, WANG XinYou

    Department of Vascular & Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To research whether peripheral blood mononuclear cells in vitro can be differentiated into endothelial progenitor cells under the induction of VEGF and bFGF. METHODS: The mononuclear cells were obtained from peripheral blood of healthy volunteers by Ficoll densitygradient centrifugation. The cells were suspended in medium 199(M199) supplemented with VEGF and bFGF for culturing ,differentiating, proliferating and identification. The expressions of specific antigens on cell surface were analysed by immunohistochemistry or immunofluorescence and the positive rate was detected by FCS. RESULTS: The mononuclear cells of human peripheral blood which were cultured and induced in vitro were positively stained for KDR, CD133 and CD34. CONCLUSION: Peripheral blood mononuclear cells can be differentiated into endothelial progenitor cells in vitro under a certain condition. ......

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