VEGF, bFGF诱导的人外周血单个核细胞向血管内皮祖细胞分化的研究
单核细胞,,单核细胞;内皮祖细胞;诱导,VEGF,bFGF诱导的人外周血单个核细胞向血管内皮祖细胞分化的研究,0引言,1材料和方法,2结果,3讨论,【参考文献】
Differentiation of peripheral blood mononuclear cells into endothelial progenitor cells in vitro induced by VEGF and bFGFSUN BaoHua, WANG Ling, YAO Qing, YI Jun, WANG Hui, WANG XinYou
Department of Vascular & Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xian 710033, China
【Abstract】 AIM: To research whether peripheral blood mononuclear cells in vitro can be differentiated into endothelial progenitor cells under the induction of VEGF and bFGF. METHODS: The mononuclear cells were obtained from peripheral blood of healthy volunteers by Ficoll densitygradient centrifugation. The cells were suspended in medium 199(M199) supplemented with VEGF and bFGF for culturing ,differentiating, proliferating and identification. The expressions of specific antigens on cell surface were analysed by immunohistochemistry or immunofluorescence and the positive rate was detected by FCS. RESULTS: The mononuclear cells of human peripheral blood which were cultured and induced in vitro were positively stained for KDR, CD133 and CD34. CONCLUSION: Peripheral blood mononuclear cells can be differentiated into endothelial progenitor cells in vitro under a certain condition. ......
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