RPHPLC法测定兔血浆中羟基喜树碱的浓度
羟基喜树碱,,羟基喜树碱；高效液相色谱-紫外检测法；血药浓度；药动学,1仪器、试药与实验动物,2方法与结果,3讨论,参考文献
摘 要 目的：建立测定羟基喜树碱在兔血浆中浓度的反相高效液相色谱-紫外检测法。方法：血浆样品酸化后，用甲醇沉淀蛋白，高速离心后取上清液进行色谱分析。色谱柱: Lichrospher C18柱(250 mm×4.6 mm，5μm),C18预柱(10 mm×4.6 mm，5μm)；流动相：ψ(甲醇∶0.01 mol/L磷酸盐缓冲液,pH4.0)=60∶40；流速：1.0 mL/min；检测波长384 nm。以5 mg/kg剂量给大耳白兔静脉推注羟基喜树碱注射液，于不同时间点采血测定药物浓度。结果：羟基喜树碱保留时间为5.0 min，定量线性范围为50~5 000 ng/mL，最低检测限为25 ng/mL。血浆中羟基喜树碱的回收率为99.88%~103.13%，日内精密度≤3.27%，日间精密度≤7.25%。羟基喜树碱静脉注射后符合二室模型。结论：本法简便实用，定量准确，可满足羟基喜树碱药动学研究的需要。关键词 羟基喜树碱；高效液相色谱-紫外检测法；血药浓度；药动学
Determination of hydroxycamptothecin concentration in rabbit plasma by RPHPLC with UV detection
CHEN Jun,FANG Yun,HUANG Lili,QIAN Xiaoping,DIAO Yuhui
(1.Department of Clinical Pharmacology;2.Department of Oncology,The Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing,Jiangsu 210008)
Abstract Objective: To establish a HPLCUV method for the determination of hydroxycamptothecin in rabbit plasma. Method: After acid treatment,plasma samples were precipitated with methanol and then centrifugated. 20μL of supernatant was injected and measured by HPLCUV method. The Chromatographic column was Lichrospher C18column:(250 mm×4.6 mm ......
您现在查看是摘要页，全文长 7385 字符。