神经肽__(期刊论文)

两种生长激素类似肽对大鼠和人流产胎儿垂体激素分泌的影响研究

http://www.100md.com 《广东药学院学报》 2005年第3期

神经肽,,神经肽；人生长激素释放激素类似肽；垂体；垂体激素,1材料和方法,2结果,3讨论,参考文献：

     摘要:目的研究ProProhGHRH(144)OH和 ProProhGHRH(144)GlyGlyCys两种神经肽对大鼠垂体或人流产胎儿垂体激素分泌的影响。方法将神经肽加入Krebs Ringer缓冲液内，在不同保温时间内测定大鼠垂体GH或人流产胎儿垂体的hGH、hTSH、hFSH、hLH和hPRL在体外的释放值。GH浓度用人GH放射免疫多抗试剂盒测定，其他垂体激素浓度用顺磁性粒子化学发光免疫测试试剂盒确定。结果鼠垂体活性结果显示，ProProhGHRH(144)OH组(0.1~10 μg/mL)和ProProhGHRH(140)GlyGlyCys组(0.01~1 μg/mL)的GH刺激值，与刺激前的空白对照值相比有明显的提高, 而且随肽浓度的增加，GH的释放量明显提高。在相同浓度下，ProProhGHRH(144)GlyGlyCys肽释放的GH值远大于ProProhGHRH(144)OH肽释放的GH值，即使ProProhGHRH(144)GlyGlyCys的剂量0.01 μg/mL，仍有较好的活性。流产胎儿垂体的体外测试结果显示，在5 μg/mL剂量下，3种多肽都产生很好的活性，ProProhGHRH (144)GlyGlyCys的平均活性是ProProhGHRH(144)OH的1.71倍，但3种活性多肽间差异没有显著性 (P>0.05)。对hTSH、hFSH、hLH和hPRL垂体激素的测试结果显示，这两种神经肽对它们没有刺激作用。结论 ProProhGHRH(144)GlyGlyCys 和 ProProhGHRH(144)OH有很好的GH释放活性和功能特异性。测试结果表明，GHRH的C端改构可调节垂体释放生长激素的活性。

    关键词:神经肽；人生长激素释放激素类似肽；垂体；垂体激素

    Effect of two novel growth hormonereleasing hormone analogs on secretion of pituitary hormones

    TANG SongShan， ZHANG JuanHui， Liu JingJing

    (Department of Biopharmaceutics, Guangdong College of Pharmacy, Guangzhou 510240, China)

    Abstract:Objective To study the influences of ProProhGHRH(144)OH and ProProhGHRH(144)GlyGlyCys peptide on secretion of pituitary hormones, rat pituitary or human abortive fetal pituitary were used to observe the release of rat GH or human GH, TSH, FSH, LH and PRL in vitro. Methods Peptide was alone added into Krebs Ringer Buffer to observe the release of GH or other human hormones on the pituitary in the different incubation durations. The rat or human GH concentrations were determined by an antiserum kit against hGH (RIA) and other human pituitary hormones, hTSH, hFSH, hLH or hPRL ......

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