醋酸棉酚对白血病HL60细胞凋亡及分化的影响
细胞凋亡,,棉酚;HL60细胞;细胞分裂;细胞凋亡;细胞分化,0引言,1材料和方法,2结果,3讨论,【参考文献】
Effects of gossypol acetic acid on apoptosis and differentiation in leukemia HL60 cellsWANG WenQing, HUANG GaoSheng, LIANG Rong, ZHANG WeiPing, WANG JuanHong, WANG Zhe, LI YuanFei, BAI QingXian, CHEN XieQun
Department of Pathology, School of Basic Medicine, Department of Hematology, Xijing Hospital, Fourth Military Medical University, Xian 710033, China
【Abstract】 AIM: To investigate the effects of gossypol acetic acid(GAA) on apoptosis and differentiation in classic human actue promylocytic leukemia cell line HL60 and study the possibility to treat leukemia with it. METHODS: The cytotoxic activity of GAA on HL60 cells was detected by means of MTT assay. Apoptosis was identified and analyzed with the help of transmission electron microscopy, cell cycle, and DNA gel electrophoresis, etc. The differentiation of HL60 cells into more mature granulocytes was detected by nitro blue tetrazolium (NBT) reduction test. RESULTS: More than 6 μmol/L GAA could suppress the proliferation and induce the apoptosis in HL60 cells in a dosedependent manner. NBT reduction test demonstrated that HL60 cells could also be induced to differentiate into more mature granulocytes by lowdose GAA (1, 4 μmol/L, P<0.05). CONCLUSION: GAA could selectively induce the apoptosis of leukemia HL60 cells in a smaller dose than that for human peripheral blood mononuclear cells, which suggests that it may be an ideal drug for leukemia therapy. ......
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