细胞分化_____过表达；NogoC；PC12

NogoC对PC12细胞分化和突起生长的影响

http://www.100md.com 《第四军医大学学报》 2006年第8期

细胞分化,,过表达；NogoC；PC12,细胞；细胞分化,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Effects of NogoC on differentiation and neurite extension of PC12 cells

    WU XiaoNa, LI Rong, LI JingWen, JU Gong

    PLA Institute of Neurosciences, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To study the effects of NogoC on the differentiation and neurite extension of PC12 cells. METHODS: The expression of NogoC in NGFtreated PC12 cells was detected using RTPCR and immunofluorescence. And pEGFPN1NogoC, the GFPtagged NogoC vector was transfected into PC12 cells. The effects of NogoC on the differentiation and neurite extension of PC12 cells were further studied by cell counting. RESULTS: A small amount of NogoC mRNA could be detected in NGFtreated PC12 cells; NogoC was found to be expressed in NGF treated PC12 cells by immunofluorescence; the neurite extension of NGFtreated PC12 cells transfected with NogoC was significantly increased according to the results of cell counting. CONCLUSION: NogoC was expressed in NGFtreated PC12 cells, and overexpressed NogoC would facilitate the differentiation and influence the neurite extension of NGF treated PC12 cells. ......

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