HPLC测定核黄素磷酸钠中核黄素的含量
核黄素磷酸钠,,核黄素；核黄素磷酸钠；HPLC,1仪器与试药,2方法与实验,3讨论,参考文献:
摘要:目的 建立核黄素磷酸钠中核黄素的含量测定方法。方法 高效液相色谱法，采用NH2色谱柱，以乙腈0.02mol/L磷酸二氢钾(体积比67∶33)用稀盐酸调pH5.3为流动相，荧光检测，激发波长：445 nm，发射波长：520 nm。结果 核黄素在0.1092～2.184 μg/mL范围与峰面积呈良好的线性关系(r=0.9999)，平均回收率为99.1%，RSD为1.5%。结论 本方法准确，灵敏，快捷。关键词:核黄素；核黄素磷酸钠；HPLC
Determination of riboflavin in riboflavin sodium phosphate by HPLC
Deng Jiexiong
(Guangzhou Institute for Drug Control，The Fourth Branch Guangzhou，510405 China)
Abstract: Objective To develop the assay methods of riboflavin in riboflavin sodium phosphate. Methods HPLC method was used to determine riboflavin in riboflavin sodium phosphate with NH2 column. The mobile phase consisted of acetonitrile-0.02 mol/L potassium dihydrogen phosphate (67∶33)(adjusted the solution with hydrochloride acid to pH 5.3). The fluorometric detector, excitation wavelength: 445 nm, emission wavelength: 520 nm. Results Riboflavin had a good linearity(r=0.9999) within 0.1092～2.184 μg/mL. The average recovery was 99.1%, with the RSD of 1.5%(n=5). Conclusions The method is accurate, sensitive and rapid. ......
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