基因治疗制剂_____α干扰素；脂质体；基因治疗制剂

αIFN质粒DNA脂质体制剂的制备及其在皮肤中表达的研究

http://www.100md.com 《广东药学院学报》 2005年第3期

基因治疗制剂,,α干扰素；脂质体；基因治疗制剂,1实验材料,2方法与结果,3讨论,参考文献：

     摘要:目的研制α干扰素(αIFN)质粒DNA脂质体制剂，为临床提供一种经病灶部位透皮给药后转基因表达抗乳头瘤病毒(HPV)的新制剂。方法利用基因克隆技术和脂质体制备技术，得到在生物体细胞内表达αIFN的表达载体，含有质粒DNA的脂质体制剂；利用ELISA方法测定在皮肤细胞层中αIFN的表达水平。结果连续3天经皮给药脂质体制剂后24 h pcIFN的表达水平最高；最后一次给药后连续5天αIFN的表达水平高于对照组(P<0.005)；没有检测到用药组和对照组动物血清中含有αIFN。结论制备得到的质粒脂质体制剂能够将质粒转染进皮肤细胞并介导体内瞬时表达。

    关键词:α干扰素；脂质体；基因治疗制剂

    Preparation of αIFN plasmid DNAliposome formulation and study on its transgenic expression in skin following topical application

    SU Weiwei，ZHONG Xueyun，ZHOU Hui，DENG Lihua，YANG Liwei，CHEN Liyun，YANG Liping，FANG Tiezheng

    (1.Sun Yatsen University，Guangzhou 510275，China；2.Jinan University，Guangzhou 510632，China)

    Abstract: Objective Preparation of αIFN plasmid DNAliposome formulation to provide a novel formulation to therapy HPV following transdermal transgenic expression. Methods With subcloning and preparation of liposome techniques，the pcIFN plasmid was obtained and the plasmidliposome formulation was made. Expression plasmid DNA for the human αIFN protein was formulated with nonionic:cationic (NC) liposomes and applied to the auricular skin of rat in single and multidose protocols. The successful transfection of perifollicular cells was quantified by ELISA. Results Skin treated for 3 days with the NC liposomes had statistically significant levels of transgenic αIFN levels present for 5 days posttreatment. Expression of transgenic αIFN was specific for areas of skin treated with NC liposomes but not control liposome. No detectable level of expressed αIFN was found in supernatant of animal blood. Conclusion The results indicate that the NC liposomes can delivery expression plasmid DNA to perifollicular cells and mediate transient transfection in vivo. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/05/31/98/04/86.htm

您现在查看是摘要页，全文长 8798 字符。