茵栀黄注射液__(期刊论文)

高效液相色谱法测定茵栀黄注射液中黄芩苷的含量

http://www.100md.com 《广东药学院学报》 2005年第2期

茵栀黄注射液,,茵栀黄注射液；高效液相色谱法；黄芩苷；含量测定,1仪器与试剂,2色谱条件,3实验方法,4讨论,参考文献

     摘要目的通过测定茵栀黄注射液中黄芩苷的含量，为其制定质量标准提供依据。方法采用高效液相色谱法，Dionex C18色谱柱，流动相为Ψ (甲醇∶水∶ 冰醋酸)=47∶53∶1，检测波长为210nm。结果黄芩苷在0.36~1.8 μg/mL范围内与峰面积呈线性关系，该制剂中黄芩苷的平均含量为1.93%，RSD=2.38%(n=3)。结论本法简便，准确，可作为该制剂质量控制的主要手段。

    关键词茵栀黄注射液；高效液相色谱法；黄芩苷；含量测定

    Determination of baicalin in Yinzhihuang injection by HPLC

    CHEN Qi ,CHEN Songhai,XU Xiaofeng

    (1.Pharmaceutical department of Guangdong Provincial Peoples hospital, Guangzhou 510080,China； 2.Department of pharmacy, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405,China)Abstract Objective To establish HPLC method for determination of baicalin in Yinzhihuang injection. Methods The HPLC method was carried on Dionex C18 column using methanolwaterglacial acetic acid (47∶53∶1) as the mobile phase and the detection wavelength of 210 nm. Results In the HPLC method the calibration curve for baicalin was linear in the range of 0.36~1.8 μg/mL. The average recovery was 97.93% with RSD=1.29%. Conclusions The method is simple, convenient and reliable for quality control of the injection ......

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