小鼠小肠_____Cajal间质细胞；ckit；小肠

阻断ckit对小鼠小肠Cajal间质细胞的影响及机制初探

http://www.100md.com 《结直肠肛门外科杂志》 2006年第1期

小鼠小肠,,Cajal间质细胞；ckit；小肠,1实验材料,2实验方法,3实验结果,4讨论,参考文献

     摘要目的：观察阻断ckit对小鼠小肠Cajal间质细胞(ICC)的影响以及与Cajal间质细胞ckit基因表达的关系。方法：选取新出生Balb/c小鼠，腹腔内隔天注射ckit抗体(ACK2)100 μg，共5次，对照组注射生理盐水，于小鼠出生后第10天观察小肠Cajal间质细胞的分布情况并检测小肠肌条ckit mRNA及蛋白的表达。结果：阻断ckit后小鼠小肠中未能明显观察到ICC分布，并且ckit mRNA表达以及ckit蛋白表达明显减少。结论：阻断ckit可导致小鼠小肠Cajal间质细胞缺失，与ICC ckit基因水平表达下调引起ICC发育受影响有关。

    关键词 Cajal间质细胞；ckit；小肠

    Exploring the Effect of Blockade of ckit on Interstitial Cells of Cajal and its Mechanisms in the Balb/c Murine Small Intestine

    Jia Houjun1, Liu Baohua1, Tong Weidong1, Zhang Lianyang1, Wen Yayuan1, Liu Ren2

    1 Department of General Surgery, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China

    2 State Key Laboratory of Trauma, Burns and Combined Injury,

    Department 2, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China

    Abstract Objective:To investigate the effect of blockade of ckit on interstitial cells of Cajal in the Balb/c murine small intestine. Methods:Newborn murine were injected intraperitoneally antibody of ckit(ACK2) 100μg every second day, and control groups were injected saline. The distribution of ICC was observed by immunohistochemistry and the expressions of ckit were measured by RTPCR and Western Blot when the murine were 10 days postpartum.Results:The distribution of ICC was lost and the mRNA and protein expression of ckit were significantly lower when ckit were blocked. Conclusion:When the cKit signal is blocked, ICC loss is found and the mechanisms of which may be related with ckit downregulated. ......

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