高效毛细管电泳__(期刊论文)

关木通中马兜铃酸A的高效毛细管电泳电导法测定

http://www.100md.com 《广东药学院学报》 2005年第2期

高效毛细管电泳,,高效毛细管电泳；电导检测器；关木通；马兜铃酸A,1仪器与试药,2方法与结果,3讨论,参考文献

     摘要目的测定关木通中马兜铃酸 A的含量。方法采用高效毛细管电泳电导检测法，研究运行缓冲液的种类、浓度、pH值、电泳电压及进样时间等对电泳的影响。结果在选定的条件下，马兜铃酸A在9.9～59.4 μg/mL浓度范围内线性关系良好，平均回收率97.3%，方法精密度(RSD)为2.39%(n=6)。结论该法可用于关木通中马兜铃酸A的含量测定。

    关键词高效毛细管电泳；电导检测器；关木通；马兜铃酸A

    Determination of aristolochic acid in Manshurian aristolochia stem by high performance capillary electrophoresis

    SONG Fenyun, WU Fuhai,ZHONG Zhaojian, DU Zhifeng

    (1. Dept.of Drug Analysis, 2. Department of Hygiene Chemistry, Guangdong College of Pharmacy, Guangzhou 510240, China)

    Abstract Objective and Method High performance capillary electrophoresis was conducted to determine the content of aristolochic acid in Manshurian aristolochia stem. The effects of buffer content, ionic strength, pH, separation voltage and electrokinetic injection on the separation efficiency were investigated. Result and Conclusion There was a linear positive relationship between the concentration of aristolochic acid and its peak height in the range of 9.9~59.4 μg/mL ......

百拇医药网 http://www.100md.com/html/DirDu/2006/05/31/98/09/77.htm

您现在查看是摘要页，全文长 7869 字符。