促血管生成素__(期刊论文)

人促血管生成素2反义真核表达载体的构建及鉴定

http://www.100md.com 《咸宁学院学报(医学版)》 2006年第1期

促血管生成素,,促血管生成素；反义真核载体；胃癌；构建,1材料和方法,2结果,3讨论,参考文献：

     摘要：目的构建人促血管生成素2(Ang2)反义真核表达载体。方法应用基因重组技术将Ang2 cDNA反向克隆到真核表达载体pEGFPN1中。结果 XholI、BamhI双酶切后，反义重组基因为5.4kb和0.726kb两条带，与理论计算相符。结论成功构建人Ang2真核表达载体，为肿瘤细胞的Ang2反义基因治疗研究创造了条件。

    关键词:促血管生成素；反义真核载体；胃癌；构建

    Construction and Identificatian of Human

    Angiopoietin2 Antisense Eukaryotic Expression Trager

    ZHU Peng ,ZHEN Chaoxin,CHEN Daoda

    (Department of Surgery, the First Affiliated Hospital of Xianning College,

    Xianning Hubei 437100,China)

    ABSTRACT:Objective To construct and identify antisense human Ang2 eukaryotic expression trager.Methods Using recombinant gene technology,we subcloned human Ang2cDNA in its antisense orientations of the pEGFPN1 trager. Results Cutting with XholI and BamHI ,antisense recombinant plasmid produce 2 bands.One was 5.4kb fragement, the other was 0.726kb fragement with corresponded to hAng2 eukaryotic expression trager.Conclusion We succeed in constructing human angiopoietin2 antisense eukaryotic expression trager. ......

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