高效毛细管电泳法测定阿莫西林口服混悬剂中阿莫西林的含量
阿莫西林;,,阿莫西林;高效毛细管电泳法;混悬剂,1仪器与试药,2方法与结果,3讨论
摘 要 目的:测定阿莫西林口服混悬剂中阿莫西林的含量。方法:高效毛细管电泳法。 毛细管60 cm×75μm,运行缓冲液30 mmol/L硼砂(pH 9.2),高压进样5 s,分离电压12 kV,温度为25 ℃,检测波长254 nm,阿魏酸为内标。结果:阿莫西林在6~30 μg/mL质量浓度范围内线性关系良好(r=0.9996),平均回收率99.10%(n=5,RSD=1.89%)。结论:本法简单、快捷、灵敏。关键词 阿莫西林;高效毛细管电泳法;混悬剂
ADetermination of amoxycillin in amoxycillin oral suspension by HPCE
GUO Dan, CHEN Nana, WANG Xiangdong, YANG Li,CHEN Zhiliang
(1. Pharmaceutical Department of Nanfang Hospital, the First Military Medical University, Guangzhou 510515; 2. Institute of Materia Medica, the First Military Medical University, Guangzhou 510515)
Abstract Objective: to determine the content of amoxycillin in amoxycillin oral suspension. Methods: HPCE was used. The best condition was achieved with a fused silca capillary tube (60 cm×75 μm), 30 mmol/L sodium tetraborate decahydrate buffer (pH 9.2), at a constant voltage of 12 kV and temperature at 25 ℃. The UV detection wavelength was at 254 nm and ferulic acid was determinaed with internal standard method. Results: the calibration curves was linear in the range of 4~20 μg/mL for amoxycillin(r=0.9997). The average recovery of amoxycillin was 99.10% (n=5 ......
您现在查看是摘要页,全文长 5756 字符。