黄芩苷_____黄芩苷;_高效液相色谱法;

HPLC法测定咳喘口服液中黄芩苷的含量

http://www.100md.com 《广东药学院学报》 2004年第3期

黄芩苷,,黄芩苷;,高效液相色谱法;,咳喘口服液,1仪器与试药,2方法与结果,3讨论,参考文献

     摘要目的：建立咳喘口服液中黄芩苷的含量测定方法。方法：用高效液相色谱法测定， Diamonsil C18色谱柱(250 mm×4.6 mm)，流动相：ψ(甲醇∶水∶磷酸)=45∶55∶0.2，检测波长280 nm。结果：黄芩苷对照品在0.1012~1.012 mg/mL范围内与峰面积呈良好线性关系；精密度、重复性试验结果RSD分别为0.42%和0.76%；平均回收率为100.47%，RSD 0.95%。结论：本法简便、灵敏、准确、重现性好, 建立的定量方法可用于咳喘口服液质量控制。

    关键词黄芩苷; 高效液相色谱法; 咳喘口服液

    Determination of baicalin in Kechuan oral liquid by HPLC

    JIN Miaozhen,LI Xiaofang,GUO Bohong,FU Jun

    (Department of Pharmacy, Guangdong College of Pharmacy， Guangzhou,Guangdong 510240)

    Abstract Objective:To establish the content determination method of baicalin in Kechuan oral liquid by HPLC.Method: The analytical column was Diamonsil C18 (250 mm×4.6 mm), ψ(methanol∶water∶phosphoric acid)=45∶55∶0.2 as mobile phase.The detection wavelength was 280nm. Result:The linear range was 0.1012~1.012 mg/mL. The RSD of the accuracy and repeatability experiment were 0.42% and 0.76% respectively. The average recovery of 100.47% with RSD=0.95%. Conclusion: The established method is convenient, sensitive, and accurate, which can be used as standard of quality control for Kechuan oral liquid. ......

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